• Multidetection HTS microplate reader with Advanced Fluorescence Polarization reading capability
• Full modularity in all detection modes covered by two matched pairs of PMT´s and optimized assay specific optical modules
• Simultaneous dual emission (SDE) detection for fastest read times and highest sensitivity
• Assay flexibility given by precise temperature control and multimode shaking capabilities

BMG LABTECH PHERAstar Plus passed the validation criteria under the following conditions:
Using BMG LABTECH's Transcreener FP Application Specific Filter Module instrument was set to 30 flashes with a positioning delay=0.1 seconds and yielded a Z' >0.7 in 1:55 min.

Optimizing Performance of the Transcreener ADP² Assay for the BMG LABTECH PHERAstar Plus HTS Microplate Reader
A detailed scientific presentation of the instrument validation.

Instrument Information
BMG LABTECH’s PHERAstar Plus is a multidetection microplate reader that combines rapid plate reading necessary for HTS with enhanced performance and sensitivity needed to measure small liquid volumes. The PHERAstar Plus reads all HTS detection modes (fluorescence intensity, time-resolved fluorescence, fluorescence polarization, luminescence, Alphascreen™, and absorbance) in all plate formats up to 1536 wells.

The PHERAstar Plus uses a unique application-specific module in conjunction with an optical reading head featuring two matched pairs of photomultiplier tubes (PMTs) that can simultaneous measure two emission signals at any desired wavelength. FP, TR-FRET and BRET are powerful detection modes that benefit from the Simultaneous Dual Emission technology. The outstanding sensitivity of the PHERAstar Plus is based on a new, innovative lens-based optical design. This design provides for outstanding sensitivity and accuracy, along with minimal read times. For FP assays, capturing both the parallel and perpendicular channels with one read dramatically decreases the inherent variability in reading the same plate twice.

BMG LABTECH’s PHERAstar Plus microplate reader provides the ideal platform for simplifying the Transcreener® ADP Assay. With its dual wavelength emission detection, the PHERAstar Plus provides the speed and sensitivity needed to take full advantage of BellBrook Labs' Transcreener® technology. Furthermore, BMG LABTECH has designed an optic module specifically for BellBrook Labs’ Transcreener®, thereby making assay setup simple.

Additional information is available from BMG LABTECH:

Detailed PHERAstar Plus information

Application note:
Transcreener^® ADP Fluorescence Polarization Assay Performed on the PHERAstar.