• Multifunctional HTS microplate reader with Simultaneous Dual Emission (SDE) detection
• Eight detection modes, Separate measurement electronics, 5 PMTs, Assay specific optic modules
• For FP the PHERAstar captures the parallel and perpendicular signal with one read using SDE
• SDE decreases read times and it increases accuracy as measured by %CV and Z' values

BMG LABTECH PHERAstar passed the validation criteria under the following conditions:
Using BMG LABTECH's Transcreener FP Application Specific Filter Module instrument was set to 50 flashes with a position delay=0.1 seconds and yielded a Z' >0.7 in 2:22 min.

Optimizing Performance of the Transcreener ADP Assay for the BMG LABTECH PHERAstar HTS Microplate Reader
A detailed scientific presentation of the instrument validation.

Note: Validation of the BMG LABTECH PHERAstar was performed using the Transcreener ADP Assay. All other instrument validations were performed using the next generation, Transcreener ADP² Assay, which contains a newer, more sensitive antibody.  Due to the fact that the BMG LABTECH PHERAstar and PHERAstar Plus have similar fluorescent polarization detection systems, the PHERAstar Plus validation is a suitable guide for the results to be expected on the PHERAstar.
PHERAstar Plus instrument validation

Instrument Information
BMG LABTECH’s PHERAstar is a multifunctional microplate reader that combines rapid plate reading necessary for HTS with enhanced performance and sensitivity needed to read small fluid volumes. The PHERAstar reads all HTS detection modes (fluorescence intensity, time-resolved fluorescence, fluorescence polarization, luminescence, Alphascreen™, and absorbance) in all plate formats up to 1536 wells. The PHERAstar uses a unique application-specific module in conjunction with an optical reading head featuring five photomultiplier tubes (PMTs) that can simultaneous measure two emission signals at any desired wavelength. This optical design provides for outstanding sensitivity and accuracy, along with minimal the read times, in simultaneous dual emission assays such as FP, FRET, and TR-FRET.  For FP assays, capturing both the parallel and perpendicular channels with one read dramatically decreases the inherent variability in reading the same plate twice.

BMG LABTECH’s PHERAstar microplate reader provides the ideal platform for simplifying the Transcreener ADP ASSAY. With its dual wavelength emission detection and five photomultiplier tubes (PMTs), the PHERAstar provides the speed and sensitivity needed to take full advantage of BellBrook Labs' Transcreener technology. Furthermore, BMG has designed an optic module specifically for BellBrook Labs’ Transcreener, thereby making assay setup simple.

Additional information is available from BMG LABTECH:

Detailed PHERAstar information

Application note:
Transcreener^® ADP Fluorescence Polarization Assay Performed on the PHERAstar.