Safire²™has been developed with the drug discovery process in mind. Due to its in-built flexibility, sensitivity and performance, Safire²™ is the ideal instrument to bridge the gap between assay development and high throughput screening. Safire²™is a fully modular microplate detection system based on the quad-4 monochromator quad-4 technology that offers a range of high speed detection techniques. In assay development and demanding research environments, where frequent changing of applications and fluorophores is required, Safire²™ eliminates the need for cumbersome filter changes thereby saving time and money.

The modular concept of Safire²™ allows upgrades to new detection modes at any time if there is a need to have access to further applications. Detection modules are available for top and bottom fluorescence intensity measurements, fluorescence polarization studies and multi-channel absorbance and luminescence measurements. Safire²™ is easily combined with a stacker module for batch processing of up to 50 microplates.

Tecan Safire²™ passed the validation criteria under the following conditions: Excitation wavelength equaled 635 nm, emission wavelength equaled 670 nm with a 20nm bandwidth; instrument was set to 1 flash and delay between move and read equaled 50 ms. These settings yielded a Z' >0.7 in 0:57 minutes

Optimizing Performance of the Transcreener ADP² Assay for the Tecan Safire²™ Microplate Reader
A detailed scientific presentation of the instrument validation.

Tecan Safire™ high performance features:

• quad-4 monochromator technology
• Autofocus - The autofocus feature further optimizes results by automatically adjusting the optics to the assay volume, microplate height or well shape (e.g. low volume plates)
• Fast ratiometric measurements: The ratio mode supports the fast change of wavelengths which is necessary for FRET assays. Quasi-simultaneous readouts in FP based on an innovative technology guarantee high speed measurements.
• Stacker – an integrated stacker module allows the processing of up to 50 microplates.
  
• High measurement speed and robotic compatibility - Safire²™ is able to handle any microplate format with up to 1536- wells with high speed in every detection mode. Multichannel luminescence and absorbance contribute in addition to the increased measurement speed. By easy integration into the Freedom EVO® series of platforms, the user has access to a wide range of throughputs.