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iuvo Chemotaxis Assay Plate

Product Cat# Size Price
iuvo Chemotaxis Assay Plate 6006 96 microchannels $200
To order, please contact us by phone at 866-313-7881, or by email at info@bellbrooklabs.com

Highly Miniaturized High-Content Assay for Neutrophil Chemotaxis in a Stable Gradient.

Chemotaxis, the movement of cells in the direction of a chemical gradient, is a fundamental process in inflammation. The iuvo Chemotaxis Assay Plate is a new approach to chemotaxis assays based on microconduit array plates designed for use with automated liquid handling and HCA platforms. The plates are constructed in a single piece with no filters or membranes and are compliant with the SBS/ANSI standard for microplates. A chemical gradient is formed at one end of the microchannel and remains stable for several hours. Microscopic imaging of cell movement into the gradient region provides quantitative data on the number of cells migrating and distance traveled. The Chemotaxis Assay Plate is currently validated for use with primary human neutrophils, with other cell models under development.

iuvoPolymorphonuclear Cell Chemotaxis Assay Protocol: iuvo™ Chemotaxis Assay Plate (PDF)

iuvo Chemotaxis Assay Plate Datasheet for neutrophil chemotaxis (PDF)

iuvo Chemotaxis Assay Plate plate

Advantages:

  • Less than 12,000 cells per data point
  • Stable gradient ≥ 3 hours
  • Addition-only assay; no inserts required
  • Compound treatment performed in the assay plate
  • Fully compatible with laboratory automation

Typical Assay Protocol

empty iuvo gradient channel iuvo gradient, wick filled iuvo gradient, cells added iuvo gradient with chemoattract added iuvo gradient, ready to be imaged
Start with an empty channel. Add media ± inhibitor. Add cells. Add chemoattractant. Incubate. Image.

Data

Image based detection of cell number and distance traveled.
iuvo inhibitor curves

Dose response inhibition of fMLP and IL-8 induced PMN chemotaxis. Approximately 12,000 cells per channel were pre-treated with the indicated amount of inhibitor in the plate for 30 min. After adding the indicated chemoattractant (200 nM fMLP or 62 nM IL-8), the plate was incubated for 2.5h. The graph shows the mean and standard deviation for replicates of 4.

Day-to-Day Reproducibility

iuvo reproducibility data

IC50 values acquired from multiple dose response experiments from different donors. PMN chemotaxis dose response inhibition curves were obtained as described above. Shown are IC50 values obtained from two separate experiments and the 95% confidence intervals as error bars.

For more information on iuvo, please visit our Technical Resources page to view our most recent posters and data sheets.