BellBrook Labs Announces New Transcreener ADP² TR-FRET Red Assay

Expanding your choices in ADP detection, the Transcreener® ADP² Assay now lets you screen kinases and other ATP-utilizing enzymes with a time-resolved-fluorescence-resonance-energy-transfer (TR-FRET) readout. For your convenience, this assay is compatible with commonly used (HTRF®) filter sets.  
 

• Universal: Any kinase, ATPase or other ADP-producing enzyme, regardless of substrate
  
• Sensitive: Z' ≥ 0.7 at less than 10% conversion; single assay for 0.1 to 1,000 µM ATP
  
• Easy: EZ protocol removes antibody optimization step
• Direct ADP Detection:  Eliminates false positives seen with technologies that use coupling enzymes prior to detection of ADP
  

The Transcreener ADP² TR-FRET Red Assay has been developed to expand your choices in ADP detection. We recognize that scientists have different preferences for fluorescence detection, and have responded by providing our Transcreener ADP² Assay in three formats: fluorescence polarization (FP), fluorescence intensity (FI) and now time-resolved-fluorescence-resonance-energy-transfer (TR-FRET). All three assays emit in the red region of the visible spectrum to minimize compound interference and come with an EZ protocol; simply titrate your enzyme and proceed to your screen.

or more information about this assay, please visit www.bellbrooklabs.com/transcreener_adp2_TR-FRET_Red.html   to view comprehensive data.

ONE ASSAY.  HUNDREDS OF TARGETS.  THAT’S SIMPLE.

KINASES
protein (tyrosine, serine/threonine)
lipid (sphingosine kinase, PI3K (α, β, Δ and γ))
carbohydrate (ketohexokinase, hexokinase, phosphofructokinase)

ATP UTILIZING ENZYMES
heat shock proteins, triphosphatases, acetyl CoA carboxylase,
glutamine synthetase, ATP citrate lyase, RecA, helicase.