Iuvo™ Chemotaxis Assay Plate

Highly Miniaturized High-Content Assay for Neutrophil Chemotaxis in a Stable Gradient.

    Ordering information

Cat# Quantity Price
6006
96 microchannels
NA
The Chemotaxis Assay Plates are now being distributed exclusively by Thermo Fisher – more information

iuvo gradient 1000Chemotaxis, the movement of cells in the direction of a chemical gradient, is a fundamental process in inflammation. The iuvo Chemotaxis Assay Plate is a new approach to chemotaxis assays based on microconduit array plates designed for use with automated liquid handling and HCA platforms. The plates are constructed in a single piece with no filters or membranes and are compliant with the SBS/ANSI standard for microplates. A chemical gradient is formed at one end of the microchannel and remains stable for several hours. Microscopic imaging of cell movement into the gradient region provides quantitative data on the number of cells migrating and distance traveled. The Chemotaxis Assay Plate is currently validated for use with primary human neutrophils with other cell models under development.

Advantages

  • Less than 12,000 cells per data point
  • Stable gradient ≥ 3 hours
  • Addition-only assay; no inserts required
  • Compound treatment performed in the assay plate
  • Fully compatible with laboratory automation

Typical Assay Protocol

iuvo schematic 1 iuvo schematic 2 iuvo schematic 3 iuvo schematic 4 iuvo schematic 5
Start with an empty channel. Add media ± inhibitor. Add cells. Add chemoattractant. Incubate. Image.

Data

sample data sm

dosecurves sm

Dose response inhibition of fMLP and IL-8 induced PMN chemotaxis. Approximately 12,000 cells per channel were pre-treated with the indicated amount of inhibitor in the plate for 30 min. After adding the indicated chemoattractant (200 nM fMLP or 62 nM IL-8), the plate was incubated for 2.5h. The graph shows the mean and standard deviation for replicates of 4.

Day-to-Day Reproducibility

iuvo gradient reproducibility sm

IC50 values acquired from multiple dose response experiments from different donors. PMN chemotaxis dose response inhibition curves were obtained as described above. Shown are IC50 values obtained from two separate experiments and the 95% confidence intervals as error bars.

 Technical Manuals

gradient 1000 data sheet icon iuvo Polymorphonuclear Cell Chemotaxis Assay Protocol: iuvo™ Chemotaxis Assay Plate (PDF)
cap thumb iuvo™ Microchannel 5250 Technical Manual (PDF)
cap thumb iuvo™ Slide 3D ICC Protocol: Immunocytochemistry Assays in 3D Extracellular Matrices (PDF)
cap thumb iuvo™ Slide 3D ICC Cell Invasion Protocol & Immunocytochemistry Staining Protocol (PDF)

Application Note

Brochures

 3DECM services data sheet icon  iuvo™ 3D Cell-Based Assay Service (PDF)
 iuvo 5250 datasheet icon  iuvo™ Microchannel 5250 (PDF)
 gradient 1000 data sheet icon  iuvo™ Chemotaxis Assay (PDF)
 iuvo 5250 datasheet icon  iuvo™ Slide – 3D ICC (PDF)
 

Scientific Posters and Presentations

SBS 2011

 

3D Tumor Cell Invasion

3d tumor poster icon An Automated High-Content Assay for Tumor Cell Migration Through 3-Dimensional Matrices (Lab Automation 2011)
3d tumor poster icon An Automated High-Content Assay for Tumor Cell Migration Through 3-Dimensional Matrices (SBS 2010, MipTec 2010)
 

3D Cell-Based Assays

3d tumor poster icon Automated High Content Assays in Biological 3D Extracellular Matrix (HCA 2011)
 

Angiogenesis

paper angiogenesis icon A Heterogeneous Angiogenesis Assay using Primary Cell Co-culture in Microchannels
 

Mammary Epithethial Cell Model

paper mammosphere icon Heterogenous Microchannel Assays Employing Primary Tumor Cells, Co-culture and 3D Matrices
 

Neutrophil Chemotaxis

chemotaxis5 poster icon Image-Based Analysis of Primary Human Neutrophil Chemotaxis in an Automated Assay (HCA 2011)
chemotaxis6 poster icon High Content Analysis of Neutrophil Phenotype During Chemotaxis (Cold Spring Harbor Automated Imaging & High-Throughput Phenotyping Meeting 2010)
iuvo labtech poster icon A New Microconduit Array Plate for Neutrophil Chemotaxis that is Compatible with High Throughput Laser Scanning Instrumentation (SBS 2010, MipTec 2010)
chemotaxis3 poster icon A Microconduit Array Plate for High Content Leukocyte Chemotaxis Assays in a Stable Gradient (SBS 2010)
chemotaxis poster icon Stable Gradients in Arrayed Microfluidic Channels for Automated Leukocyte Chemotaxis Assays