Iuvo™ Chemotaxis Assay Plate
Highly Miniaturized High-Content Assay for Neutrophil Chemotaxis in a Stable Gradient.
The Chemotaxis Assay Plates are now being distributed exclusively by Thermo Fisher – more information
Chemotaxis, the movement of cells in the direction of a chemical gradient, is a fundamental process in inflammation. The iuvo Chemotaxis Assay Plate is a new approach to chemotaxis assays based on microconduit array plates designed for use with automated liquid handling and HCA platforms. The plates are constructed in a single piece with no filters or membranes and are compliant with the SBS/ANSI standard for microplates. A chemical gradient is formed at one end of the microchannel and remains stable for several hours. Microscopic imaging of cell movement into the gradient region provides quantitative data on the number of cells migrating and distance traveled. The Chemotaxis Assay Plate is currently validated for use with primary human neutrophils with other cell models under development.
- Less than 12,000 cells per data point
- Stable gradient ≥ 3 hours
- Addition-only assay; no inserts required
- Compound treatment performed in the assay plate
- Fully compatible with laboratory automation
Typical Assay Protocol
|Start with an empty channel.||Add media ± inhibitor.||Add cells.||Add chemoattractant. Incubate.||Image.|
Dose response inhibition of fMLP and IL-8 induced PMN chemotaxis. Approximately 12,000 cells per channel were pre-treated with the indicated amount of inhibitor in the plate for 30 min. After adding the indicated chemoattractant (200 nM fMLP or 62 nM IL-8), the plate was incubated for 2.5h. The graph shows the mean and standard deviation for replicates of 4.
IC50 values acquired from multiple dose response experiments from different donors. PMN chemotaxis dose response inhibition curves were obtained as described above. Shown are IC50 values obtained from two separate experiments and the 95% confidence intervals as error bars.
iuvo™ Chemotaxis Assay Plate
- Technical Manual – iuvo™ Polymorphonuclear Cell Chemotaxis Assay Protocol: iuvo™ Chemotaxis Assay Plate (PDF)