The Transcreener AMP²/GMP² Assay allows you to detect any AMP²/GMP² producing enzyme (e.g., ligases, synthetases, phosphodiesterases) using any precursor substrate, including cAMP, cGMP, ATP, or NAD. The method is simple and HTS-proven: run your enzyme reaction, add detection reagents, and read the far red FP signal on any multimode plate reader.

XMP enzyme products, including AMP, GMP and CMP displace a fluorescent tracer from a highly selective antibody resulting in a decrease in fluorescence polarization. The assay uses a far red tracer, which reduces compound interference and provides a robust ratiometric readout.

One Assay ... Hundreds of targets

• Ligases: Ub and SUMO Ligases, DNA and RNA Ligases
• Synthetases: Acyl CoA Synthetase, Amino-Acyl tRNA Synthetases, NAD Synthetase
• Phosphodiesterases: cAMP, cGMP
• Sialyltransferases: (CMP detection)
• Protein deAMPylase
• SINGLE ADDITION: one-step, simple mix and read format.
• STABLE: reagents and signal are stable > 24 hour
• ROBUST: Z’ > 0.7 for initial velocity reactions
• LESS INTERFERENCE: Far red fluorescence minimizes compound interference.