TRANSCREENER UDP2 Assay

Universal UDP-Glycosyltransferase Assay

 

The Transcreener UDP2 Assay is a single step, homogenous, fluorescent assay for detection and screening of UDP-producing enzymes. Direct detection of UDP with a fluorescence polarization (FP) readout provides a safe, HTS-compatible alternative to cumbersome radioassay methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product. Based on BellBrook's proprietary nucleotide immunodetection technology, the Transcreener UDP2 Assay is compatible with any enzyme class that produces UDP, including UDP-glucose-, UDP-galactose- and UDP-glucuronosyl-transferases as well as glycogen, hyaluranon and cellulose synthases. 

transcreener udp fp

Direct detection of UDP with no coupling enzymes means
greater sensitivity with less interference.
 

Robust, HTS-Compatible Assay

  • Simple, “mix and read” assay format.
  • Far red fluorescence minimizes interference.
  • Outstanding deck and signal stability.
  • Compatible with any multimode plate reader.
     

More Sensitive

New antibody provides greater sensitivity than orignal Transcreener UDP Assay: excellent signal and Z’ using less than 1μM UDP-sugar.