Posters and Presentations

 

 

Transcreener HTS Assays          

iUVO Microarray Conduit Platform

Transcreener® ADP2 Assay Air-Liquid Interface (ALI)
Transcreener® ADP Assay SBS 2011
Transcreener® AMP/GMP Assay 3D Tumor Cell Invasion
Transcreener® GDP Assay 3D Cell-Based Assays
Transcreener® UDP Assay Angiogenesis
Transcreener® EPIGEN Assays Mammary Epithethial Cell Model

 

Neutrophil Chemotaxis

 

Transcreener® ADP2 Assay

  1. NEW! Determination of Drug Residence Time in an HTS Format using Kinetic Analysis with the Transcreener® ADP2 Assay (SLAS 2015)
  2. Transcreener® ADP2 Assay: Simplifying and Streamlining Kinase Profiling from Experimental Design through Data Analysis (SLAS 2014)
  3. Comparison of ADP Detection Methods used for High Throughput Screening (SLAS 2013)
  4. Transcreener® ADP2Assays: Streamlining Inhibitor Profiling for Kinases with Diverse ATP Requirements (MIPTEC 2010)
  5. ADP Detection in FP, TR-FRET and FI Assay Formats: Versatile Kinase/ATPase Detection Without the Use of Coupling Enzymes (SBS 2010)
  6. ADP Detection in FP, TR-FRET and FI Assay Formats: Versatile Kinase/ATPase Detection without the Use of Coupling Enzymes (Screening Europe, 2010)
  7. Utility of Hybrid Multi-Mode Readers using Both Monochromator- and Filter-Based Detection Capabilities as Demonstrated with Fluorescence Polarization, Time-Resolved Fluorescence Resonance Energy Transfer, and Fluorescence Intensity Assays for the Measurement of ADP (Lab Auto 2010)
  8. Improved Versatility of the Transcreener® ADP2 Assay: Alternative Fluorescence Detection Modes for All Major Instrument Platforms (Protein Kinase Target, 2009)
  9. Transcreener® ADP2 TR-FRET Red Assay: Homogeneous ADP Detection (SBS 2009)
  10. Targeting Low Activity ATP-utilizing Enzymes with the Improved Transcreener® ADP2 FP Assay (SBS 2009)
  11. Detecting ATP-utilizing Enzyme Activity Using a Simple Fluorescence Intensity Quenching ADP Assay (SBS 2009)
  12. Robust Detection of ATP-Utilizing Enzymes with an Improved Fluorescence Polarization ADP Detection Assay (Screening Europe 2009)
  13. Volume Scaling of the Transcreener® ADP2 FP Assay on the BioTek Synergy™ 2 and 4 Multi-Mode Microplate Readers Allows For Easy Transition From Assay Development to High-Throughput Screening (ALA 2009)
  14. Generating Better Biology Through the Use of BellBrook Labs’ Transcreener® ADP2 FP Assay and the BMG LABTECH PHERAstar Plus (ALA 2009)
  15. Demonstration of the Next Generation Transcreener® ADP2 FP Assay on the Tecan Safire2™ Microplate Reader (ALA 2009)
  16. Generate Improved Fluorescence Polarization Screening Results on PerkinElmer’s EnVision® Microplate Reader Through the Use of the Transcreener® ADP2 FP Assay (ALA 2009)

 

Transcreener® ADP Assay

  1. Beyond Kinases: Interrogating the Purinome (Keystone 2010)
  2. Optimizing Performance of the Transcreener ADP Assay for Commonly Used Multiwell Readers (SBS 2008)
  3. Application of the Transcreener Kinase Assay Platform in Evaluating PI3 Lipid Kinases (SBS 2007)
  4. Expanding Transcreener Assay Technology: ADP Detection using TR-FRET
  5. Interrogating Diverse Target Families by Homogenous Immunodetection of Nucleotides (Glyco 2007)
  6. Interrogation of Protein Phosporylation, Ubiquitination and Sumoylation Pathways Using Adenine Nucleotide Detection Assay (cell Signaling 2007)
  7. ADP Detection Assay for Diverse Kinases and ATPases
  8. A Robust and Flexible HTS Kinase Assay Using ADP Detection
  9. Simplifying Kinase Profiling using ADP Detection with the Transcreener Kinase Assay
  10. Screening Covalent Regulatory Enzymes: Glycosyltransferases, Sulfotransferases, Phosphodiesterases and other Emerging Targets (powerpoint of tutorial presented at SBS 2006)

 

Transcreener® AMP/GMP Assay

  1. NEW! TR-FRET Based Transcreener AMP2/GMP2 Assay for Screening Ligases, Synthetases and Phosphsdiesterases (SLAS 2014)
  2. Monitoring flux through the Sumoylation pathway using the Transcreener AMP/GMP assay (Keystone 2009)
  3. Application of theTranscreener AMP/GMP Assay for Interrogating Ubiquitinylation Cascades (SBS 2008)
  4. Homogenous AMP and GMP Detection : Applications in Drug Discovery (SBS 2007)
  5. Interrogation of Protein Phosporylation, Ubiquitination and Sumoylation Pathways Using Adenine Nucleotide Detection Assay (Cell Signaling 2007)
  6. Transcreener PDE Assay: Homogenous AMP and GMP Detection
  7. Screening Covalent Regulatory Enzymes: Glycosyltransferases, Sulfotransferases, Phosphodiesterases and other Emerging Targets (powerpoint of tutorial presented at SBS 2006)
  8. Screening Small Molecule Regulatory Enzymes: Glycosyltransferases, Sulfotransferases and Phosphotransferases

 

Transcreener® GDP Assay

  1. NEW! A High Throughput Assay for Rho Guanine Nucleotide Exchange Factors Based on the Transcreener® GDP Assay (FASEB Small GTPases 2015)
  2. Selective Detection of LRRK2 GTPase Activity with the Transcreener® GDP Assay (SLAS 2013)
  3. Investigation of different GTPase classes with the Transcreener GDP Assay (SLAS 2012)
  4. Discovery of small molecule inhibitors for RGS proteins using the RGS screen Platform (SBS 2011)
  5. Targeting RGS Proteins using Engineered Galpha Proteins and the Transcreener® GDP Assay (SBS 2010)
  6. Engineering G alpha Proteins for Detection of RGS Protein GAP Activity with the Transcreener® GDP Assay (Screening Europe 2009)
  7. GDP Detection Using the Transcreener HTS Assay Technology (SBS 2008)
  8. An RGS Protein HTS Strategy using the Transcreener GDP Assay (SBS 2008)

 

Transcreener® UDP Assay

  1. NEW! Discovery of Inhibitors of GALNT3 by Orthogonal Pooled Screening with a Universal Glycosyltransferase Assay (Glyco 2015)
  2. Development of a Homogenous Transcreener® UDP2 TR-FRET Assay for High Throughput Screening of Glycosyltransferases (DDD 2015)
  3. Screening Diverse Glycosyltransferases Using Homogenous, Fluorescent Transcreener HTS Assays For Direct Detection of Nucleotide Products (SLAS 2014)
  4. Transcreener® High Throughput Screening Assays for Glycosyltransferases (SLAS 2013)
  5. Interrogating Diverse Target Families by Homogenous Immunodetection of Nucleotides (Glyco 2008)
  6. Homogenous UDP Detection: Applications in Glycobiology (Glyco 2007)
    Note: the following posters include data generated with the Transcreener UGT 488Assay. This assay has been replaced by the Transcreener UDP far red Assay, which minimizes assay interference while adhering to our strict assay standards
  7. Screening Covalent Regulatory Enzymes: Glycosyltransferases, Sulfotransferases, (SBS 2006)
  8. Phosphodiesterases and other Emerging Targets (powerpoint tutorial presented at SBS 2006)

 

Transcreener® EPIGEN Assays

  1. NEW! Optimization of Enzyme Reactions for Screening and Profiling Wild Type EZH2 with the Transcreener EPIGEN Methyltransferase Assay (SLAS 2014)
  2. Profiling MT Inhibitors with Transzyme™ Methyltransferase Enzyme Assay Kits (SLAS 2013)
  3. Validation of  Transcreener® EPIGEN  Assays for Screening Chromatin Modifying Enzymes: Methylation and Acetylation (SLAS 2012)
  4. Automation of a Generic Fluorescence Methyl transferase activity assay (SLAS 2012)
  5. Development and validation of generic fluorescence methyltransferase assay using the Transcreener AMP GMP assay (SBS 2011)
  6. Development and validation of generic fluorescence acetyltransferase assay using the Transcreener AMP GMP assay (SBS 2011)

 

iUVO Microarray Conduit Platform

 Air-Liquid Interface (ALI)

  1. An HTS-Compatible Plate For Highly Miniaturized Cultures Of Primary Human Bronchial Epithelial Cells At Air-Liquid Interface (FASEB Small GTPases 2015)

 

 SBS 2011

  1. Automated High Content Assays in Biological 3D Extracellular Matrix
  2. Image-Based Analysis of Primary Human Neutrophil Chemotaxis in An Automated Assay
  3. Development of Optimized 3D Migration and Invasion Assays for siRNA Screening (In Collaboration with the University of Wisconsin - Madison Graduate Program in Cellular and Molecular Biology and Department of Pharmacology).

 

3D Tumor Cell Invasion

  1. An Automated High-Content Assay for Tumor Cell Migration Through 3-Dimensional Matrices (Lab Automation 2011)
  2. An Automated High-Content Assay for Tumor Cell Migration Through 3-Dimensional Matrices (SBS 2010, MipTec 2010)

 

3D Cell-Based Assays

  1. Automated High Content Assays in Biological 3D Extracellular Matrix (HCA 2011)

 

Angiogenesis

  1. A Heterogeneous Angiogenesis Assay using Primary Cell Co-culture in Microchannels

 

Mammary Epithelial Cell Model

  1. Heterogenous Microchannel Assays Employing Primary Tumor Cells, Co-culture and 3D Matrices

 

Neutrophil Chemotaxis

  1. Image-Based Analysis of Primary Human Neutrophil Chemotaxis in an Automated Assay (HCA 2011)
  2. High Content Analysis of Neutrophil Phenotype During Chemotaxis (Cold Spring Harbor Automated Imaging & High-Throughput Phenotyping Meeting 2010)
  3. A New Microconduit Array Plate for Neutrophil Chemotaxis that is Compatible with High Throughput Laser Scanning Instrumentation (SBS 2010, MipTec 2010)
  4. A Microconduit Array Plate for High Content Leukocyte Chemotaxis Assays in a Stable Gradient (SBS 2010)
  5. Stable Gradients in Arrayed Microfluidic Channels for Automated Leukocyte Chemotaxis Assays