Bellbrook Labs

Biology at Work

Subscribe to product updates

Transcreener® ADP2 Assays

Still Universal. Now More Sensitive. New TR-FRET and FI Detection Modes.

Product Cat# Size Price
Transcreener ADP2 FP Assay 3010-1K 1000 assays (384 well format) $585
Transcreener ADP2 TR-FRET Red Assay 3011-1K 1000 assays (384 well format) $675
Transcreener ADP2 FI Assay 3013-A 200 assays (96 well format) $290
Transcreener ADP2 FI Assay 3013-1K 1000 assays (384 well format) $585
  Sufficient reagents are provided to complete up to 200 assays (3013-A; 96 well format) or 1000 assays ( 3010-1K and 3013-1K; 384 well format). The exact number of assays is dependent on your enzyme reaction conditions. If you are using > 100 μM ATP, custom packaging is available.
Bulk Ordering      
For orders of 10,000 wells or more, please contact us for a price quote. info@bellbrooklabs.com

Transcreener ADP2 Assays are homogenous assays with fluorescent readouts that enable the facile detection and screening of established drug targets including protein and lipid kinases as wells as emerging targets such as carbohydrate kinases, tryphosphatases, heat shock proteins and other ATPases. The assay is based on the immunodetection of ADP and allows the screening of diverse enzymes with native and synthetic substrates, or enzymes with intrinsic ATPase (no substrate) – all with the same set of reagents.

Features:

20X MORE SENSITIVE

  • More sensitive antibody means a single assay provides Z' Values ≥ 0.7 from 0.1 to 1,000 µM ATP
  • Broad ATP range enables identification of ATP competitive or allosteric inhibitors
  • Use less enzyme without compromising Z' values at low ATP conversion
  • Use with any kinase, ATPase or other ADP-producing enzyme, regardless of substrate

TURN-KEY PROTOCOL

  • EZ protocol eliminates antibody optimization step
  • Predetermined reagent concentration for a broad range of ATP concentrations
  • Simply titrate your enzyme then proceed to your screen

VALIDATED

THREE DETECTION MODES

  • Fluorescence Polarization (FP)
  • Time-Resolved Forster-Resonance Energy Transfer (TR-FRET)
  • Fluorescence Intensity (FI)

To accommodate the variety of instrument and detection format preferences expressed by our customers, we have expanded the Transcreener ADP2 Assay product line to include three fluorescent detection formats, all with emission in the red region of the visible spectrum to minimize compound interference. All three assays share the same qualities of assay sensitivity and ease of use. Whether you are doing assay development, screening, or hit to lead activities, there is a Transcreener ADP2 Assay that is right for you.

For more information on the Transcreener ADP2 Assays contact BellBrook Labs:
866.313.7881 in North America
info@bellbrook labs.com