Ordering Information

Product	Cat#	Quantity	Price
Transcreener ADP2 TR-FRET
	3013-A	200 assays (96-well)	$290
	3011-1K	1,000 assays (384-well)	$675
	If you are using > 100 μM ATP, custom packaging is available.
	For Bulk Orders (10,000 wells +), please contact us (info@bellbrook labs.com) for a quote.

 

Universal ADP Detection with a Far-Red TR-FRET Readout ...

The Transcreener® ADP2 TR-FRET Red Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes. It is a simple, one-step homogenous detection assay that accomodates a broad range of ATP concentrations (0.1 to 1,000 µM ATP) and produces excellent signal (Z´ ≥ 0.7 at 10% conversion of 1 µM ATP).

Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.

• UNIVERSAL: Detect any ADP-producing enzyme using any substrate
• SINGLE ADDITION: one-step, simple mix and read format.
• SENSITIVE: Z' ≥ 0.7 at low ATP conversion.
• LOW INTERFERENCE: far red, time resolved signal.