Biology at Work
Transcreener® AMP/GMP Assay- far red FP
Note: prior to 4/1/08 The Transcreener AMP/GMP Assay was known as the Transcreener PDE Assay.
| Product | Cat# | Size | Price |
|---|---|---|---|
| Transcreener AMP/GMP Assay | 3006-1K | 1,000 assays | $585 |
| This is an evaluation kit. Sufficient reagents are provided to complete up to 1000 assays. The exact number of assays is dependent on your enzyme reaction conditions. | |||
| Customized Bulk Ordering | |||
| For orders of 10,000 wells or more, please contact us for a price quote. info@bellbrooklabs.com | |||
Click here for valuable instrument optimization information.
- Transcreener AMP/GMP Technical Manual
- Transcreener AMP/GMP Posters and Presentations
- Transcreener Technical References
Benefits
- Single-step reaction using unlabeled, native cAMP, cGMP and ATP substrates
- No "in-the-dark" steps. No manual steps. No secret reagents.
- Wide substrate concentration range [ 1 μM to 1000 μM]
- Real-time or endpoint detection
Description
Phosphodiesterases control a variety of cellular processes by hydrolyzing the second messenger signaling molecules cAMP and cGMP. The Transcreener AMP/GMP Assay is a far-red, competitive fluorescence polarization immunoassay that detects the reaction products AMP or GMP. Enzyme reaction progress is indicated by a decrease in the fluorescence polarization signal. The Transcreener AMP/GMP Assay is a simple two-step, endpoint assay accomodating cAMP/cGMP concentrations of 0.1 to 10 μM with a single reagent mix (up to 1 mM substrate has been used). The assay provides an excellent signal under initial velocity conditions resulting in overall Z’> 0.6. Although originally designed for PDEs that hydrolyze cAMP or cGMP, the Transcreener AMP/GMP Assay can be used for any enzyme class that produces either AMP or GMP (eg: a ligase reaction which converts ATP to AMP). Reduced development costs and accelerated drug discovery are achieved by utilizing this one simple streamlined screening method.
The Transcreener AMP/GMP Detection Mixture comprises an AMP/GMP Alexa633 tracer bound to an AMP/GMP antibody, which is displaced by AMP or GMP, the products generated during PDE reactions. The displaced tracer freely rotates leading to a decrease in fluorescence polarization, relative to the bound tracer. Therefore, AMP/GMP production creates a proportional decrease in polarization values.
For additional information on the Transcreener AMP/GMP Assay
contact BellBrook Labs:
866.313.7881 in North America
Info@bellbrooklabs.com
