Ordering Information

Product	Readout	Cat#	Quantity	Price
Transcreener GDP Assay	FP	3009-1K	1,000 assays (384-well)	$614
	FI	3014-A	200 assays (96-well)	$305
		3014-1K	1,000 assays (384-well)	$614
	For Bulk Orders (10,000 wells +), please contact us (info@bellbrook labs.com) for a quote.

 

The Only Mix-and-Read Fluorescent GTPase Assay ... The Transcreener GDP Assays are single step, homogenous, fluorescent assays for direct detection and screening of GTPases. Available with fluorescence polarization (FP) and fluorescence intensity (FI) readouts, they provide a safe, HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than colorimetric phosphate detection methods. Based on BellBrook's proprietary nucleotide immmunodetection technology, the Transcreener GDP Assays are compatible with any enzyme class that produces GDP, including monomeric small G proteins, such as CDC42, and Ga subunits of heterotrimeric G proteins, such as Gai1.

GDP displaces tracer from a highly selective monoclonal antibody resulting in a decrease in fluorescence polarization or an increase in fluorescence intensity.

DETECT ANY GDP-PRODUCING ENZYME: GTPases, fucosyltransferases, mannosyltransferases

TURNKEY PROTOCOL: Run enzyme reaction, add detection reagents, read plates

TWO READOUTS AVIALABLE: Fluorescence polarization and fluorescence intensity, both far red