Transcreener^® Instrument Validation Program

BellBrook Labs has designed an instrument validation program in an effort to align the high standards we have set for our assay to your specific instrument. It is our goal that you be as successful as possible using Transcreener assays in as little time as possible. Our scientists personally test each line of instruments and are able to offer you both confidence that an instrument will perform well with our far red FP assays and a guideline for setting up your instrument.

Look for these stickers on instruments that have successfully met the validation criteria.

Validation Criteria

• 384-Well Format
• Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
• Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
• Z' and Δ mP specifications to be met using Transcreener ADP² Assay reagents
• Read time to achieve Z' and Δ mP specifications ≤ 5 minutes

		Transcreener® Assay
		ADP2 FP and GDP FP	ADP2 FI and GDP FI	ADP2 TR-FRET	AMP/GMP FP	UDP FP	ADP FP (old product)
TECAN	Safire²™	√	√	√	data avail	data avail	√
	Infinite® M200		√
	Infinite® F200		√
	Infinite® F500	√					√
	Infinite® M1000	√	data avail				√
PerkinElmer	EnVision®	√	√	√	√		√
BMG LABTECH	PHERAstar		√				√
	PHERAstar Plus	√	√	√	data avail		√
	FLUOstar Omega		√
	POLARstar Omega	X (96 well)	√
Molecular Devices	Analyst® GT	√					√
	SpectraMax M2	n/a	data avail	n/a	n/a	n/a	n/a
BioTek	Synergy™ 2	√					√
	Synergy™ 4	√					√
	Synergy™ H4	√	√	√