Transcreener^® Assays - Instrument Validation

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

		Transcreener ® Assays
		FP Assays ADP2, AMP/GMP, GDP, UDP	FI Assays ADP2, GDP	TR-FRET Assays ADP2
BioTek	Synergy™ H4/2/4	validated	validated	validated
BMG LABTECH	POLARstar Omega	validated	validated	validated
	FLUOstar Omega	not capable	validated	validated
	PHERAstar Plus/FS	validated	validated	validated
Molecular Devices	Analyst® GT/HT	validated	validated	validated
	Gemini® XPS/EM	not capable	validated	not capable
	SpectraMax® M2/M2e	not capable	validated	not capable
	SpectraMax® M5/M5e/FlexStation® 3	not validated	validated	validated
PerkinElmer	EnVision®/EnVision® Xcite	validated	validated	validated
TECAN	Infinite® M1000/Safire2™	validated	validated	contact us
	Infinite® M200	not capable	validated	not capable
	Infinite® F500	validated	validated	not validated
	Infinite® F200/Ultraevolution	contact us	validated	contact us

Validation Criteria

• 384-Well Format
• Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
• Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
• Z' and Δ mP specifications to be met using Transcreener ADP² Assay reagents
• Read time to achieve Z' and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.