Assay Development Services Include:
- Target Protein Sourcing/Production – If the target protein is available from the customer or a vendor, we will test it for purity and functional activity; if not, we will produce it using the most efficient and cost effective expression and purification strategy.
- Assay Optimization with Target Protein – We will determine the optimal buffer composition, enzyme concentration, and reaction time and optimize detection reagents to produce the maximal signal under initial velocity conditions
Case Study: Probing p97 ATPase with the Transcreener® ADP2 Assay
- Assay Validation – Depending on customer needs, validation could include dose responses with control inhibitors, determination of kinetic parameters, pilot screens with targeted libraries, or interference screens.
- Protein Kinases
- Lipid Kinases
- Carbohydrate Kinases
- GTPases, GAPs, GEFs