Choose The Right Assay Plate for Your Experiment

Transcreener® HTS Assays rely on direct immunodetection of nucleotides with a far-red readout in a simple mix-and-read format.  The assays are available in three different detection modes, fluorescence polarization (FP), fluorescence intensity (FI), and time-resolved Forster resonance energy transfer (TR-FRET).  A good microplate reader and the right choice of plates to differentiate specific signal versus background signal or “noise” is crucial to the success of these assays.

To achieve the best results be sure to:

  • Use polystyrene, non-binding plates
  • Make sure the plate has the correct working volume
  • Use black plates for FP & FI
  • Use white plates for TR-FRET

FP & FI Assay Plates

384-Well Low Volume Black Assay Plates

4514 Corning 384-Well Assay Plate Black

Corning® 384-well black plates are a great fit for Transcreener FP and FI assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 20 µL for these plates. Black plates absorb light and reduce background as well as well-to-well cross talk. Square well tops enable easier robotic handling, while round bottoms minimize trapped air and provide better assay Z'. Polystyrene non-binding services (NBS) is Corning's proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.

Product Details 
Number of Wells384
Plate Color
Black
Surface CharacteristicsNon-Binding (NBS) Coated
BrandCorning #4514
Plate FeaturesSquare Wells, Round Bottom
Maximum Well Volume35 µL
Recommended Working Volume2-20 µL
CompositionTreated Polystyrene
Plate LidsNot Included
SterileNo

96-Well Half Area Black Assay Plates

Corning 96-Well Assay Plate Black and White

Corning® 96-well half area plates are a great fit for Transcreener FP and FI assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 50 µL for these plates. Black plates absorb light and reduce background as well as well-to-well cross talk. Polystyrene non-binding services (NBS) is Corning's proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.

Product Details 
Number of Wells96
Plate Color
Black
Surface CharacteristicsNon-Binding (NBS) Coated
BrandCorning #3686
Plate FeaturesRound Wells, Flat Bottom
Maximum Well Volume190 µL
Recommended Working Volume25-125 µL
CompositionTreated Polystyrene
Plate LidsNot Included
SterileNo

Ordering Information

TR-FRET Assay Plates

384-Well Low Volume White Assay Plates

Corning® 384-well white plates are a great fit for Transcreener TR-FRET assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 20 µL for these plates. Square well tops enable easier robotic handling, while round bottoms minimize trapped air and provide better assay Z'. Polystyrene non-binding services (NBS) is Corning's proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.

Product Details 
Number of Wells384
Plate Color
White
Surface CharacteristicsNon-Binding (NBS) Coated
BrandCorning #4513
Plate FeaturesSquare Wells, Round Bottom
Maximum Well Volume35 µL
Recommended Working Volume2-20 µL
CompositionTreated Polystyrene
Plate LidsNot Included
SterileNo

96-Well Half Area White Assay Plates

Corning 96-Well Assay Plate Black and White

Corning® 96-well half area plates are a great fit for Transcreener TR-FRET assays. They feature low working volume to conserve precious enzyme. BellBrook Labs recommends a final volume of 50 µL for these plates. Polystyrene non-binding services (NBS) is Corning's proprietary treatment which limits protein binding at low concentrations. The microplate is non-sterile and comes without a lid. However, for long incubation periods, BellBrook Labs recommends covering your sample wells to reduce evaporation.

Product Details 
Number of Wells96
Plate Color
White
Surface CharacteristicsNon-Binding (NBS) Coated
BrandCorning #3642
Plate FeaturesRound Wells, Flat Bottom
Maximum Well Volume190 µL
Recommended Working Volume25-125 µL
CompositionTreated Polystyrene
Plate LidsNot Included
SterileNo

Ordering Information

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