Choose The Right Assay Plate for Your Experiment
Transcreener® HTS Assays rely on direct immunodetection of nucleotides with a far-red readout in a simple mix-and-read format. The assays are available in three different detection modes, fluorescence polarization (FP), fluorescence intensity (FI), and time-resolved Forster resonance energy transfer (TR-FRET). A good microplate reader and the right choice of plates to differentiate specific signal versus background signal or “noise” is crucial to the success of these assays.
To achieve the best results be sure to:
- Use polystyrene, non-binding plates
- Make sure the plate has the correct working volume
- Use black plates for FP & FI
- Use white plates for TR-FRET