Looking Ahead: POLQ in the Future of Immuno-Oncology
Tuesday, 06 September 2022
The most well-known and widely studied mechanisms of double-stranded DNA break (DSB) repairs are homologous recombination (HR) and classical non-homologous end joining (C-NHEJ). HR is almost error-free due to formatting by the homologous sister chromosome. C-NHEJ relies on direct ligation of double-stranded DNA ends and only ever presents errors at the junction points. Recently, a
- Published in Emerging Targets, HTS Assays, Innate Immunity
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Discovering the Emerging Importance of PARG in Immunity
Monday, 08 August 2022
Poly (ADP-ribose) glycohydrolase (PARG), along with poly (ADP-ribose) polymerase 1 (PARP1) are the principal elements of the DNA damage response (DDR). When single-strand breaks occur in cellular DNA, PARP1 mediates the poly ADP ribosylation of itself and target proteins, such as histones, promoting the decompaction of chromatin and recruiting relevant enzymes to initiate DNA repair.
- Published in Emerging Targets, HTS Assays, Innate Immunity
Helicases as Powerful Tools in Innate Immunity
Monday, 11 July 2022
Helicases are among the largest and most highly conserved families of enzymatic proteins in eukaryotic organisms. These proteins utilize NTP hydrolysis (usually ATP) to drive their recognition, remodeling, and response to target DNA or RNA.1 Nearly every aspect of nucleic acid metabolism is mediated by helicases. DNA helicases function in replication, repair, recombination, transcription, chromosome
- Published in Emerging Targets, HTS Assays, Innate Immunity
OAS1: The Cost of Host Defense
Monday, 13 June 2022
OAS1 (Oligoadenylate synthetase 1) is induced by type 1 interferon signaling. It recognizes 18 bp (or longer) double stranded RNA segments from invading viruses in the cytosol and catalyzes the production of 2’-5’ linked oligoadenylate (2-5A) from ATP. The 2-5A then, at the expense of yet more ATP, exclusively activates endoribonclease L (RNase L) by
- Published in Emerging Targets, HTS Assays, Innate Immunity, Products
DDX41 as a Sensor, Suppressor, and Modulator
Thursday, 26 May 2022
DDX41 [DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 41] is a cytosolic helicase sensor for dsDNA, DNA/RNA complexes, and cyclic dinucleotides (CDNs). Its N-terminal domain is responsible for translocation from the cytoplasm to the nucleus. Its DEAD domain, with its signature aspartate-glutamate-alanine-aspartate motif, is important for ATP-powered DNA/CDN detection and signaling. The remaining C-terminal domain functions as a
- Published in Emerging Targets, HTS Assays, Innate Immunity
The Role of ALPK1 in Health and Disease
Thursday, 12 May 2022
ALPK1 (Alpha Kinase 1) is an atypical serine/threonine-protein kinase that specifically detects and binds the pathogen-associated pattern metabolites (PAMPs), ADP-beta-D-manno-heptose (Beta-ADP-Heptose) or D-glycero-beta-D-manno-heptose 1,7-bisphosphate (HBP). These metabolic precursors of lipopolysaccharide (LPS) biosynthesis are present in all Gram-negative and some Gram-positive bacteria. This interaction stimulates ALPK1 to phosphorylate and activate TIFA, initiating an innate immune response
- Published in Emerging Targets, HTS Assays, Innate Immunity
Resolving the Many Roles of DHX36
Wednesday, 27 April 2022
While most researchers are familiar with canonical DNA structures, primarily B (but also even A or Z) DNA, an even more exotic form exerts a vast influence over nearly every aspect of nucleic acid function. In both DNA and RNA, tracts of guanine with at least four consecutive members can self-associate via Hoogsten base pairing
- Published in Emerging Targets, HTS Assays, Innate Immunity
MAPK1, An Elusive Regulator of Innate Immunity
Wednesday, 23 February 2022
Mitogen-Activated Protein Kinases (MAPKs) and MAPK Signaling Cascade Mitogen-activated protein kinases (MAPKs) are Ser/Thr kinases involved in converting extracellular signals to a large spectrum of fundamental cellular processes such as apoptosis, cell division, metabolism survival, etc. This signaling cascade is known as the MAPK pathway. MAPKs are highly conserved among eukaryotes, which mirrors its indispensable
- Published in HTS Assays, Innate Immunity
The Double-Edged Sword in Cancer Pathogenesis – JNK1
Wednesday, 03 November 2021
Properties and Function of JNK1 c-Jun N-terminal kinase 1 (JNK1), also commonly known as Mitogen-Activated Protein Kinase 8 (MAPK8), is a member of the JNK subgroup of MAP kinases. JNK1 is encoded by the MAPK8 gene. MAP kinases form a superfamily of protein kinases that function by phosphorylating various protein substrates, including transcription factors, which
- Published in Emerging Targets, HTS Assays, Innate Immunity
Webinar: Targeting Kinases Involved in the Innate Immune Response
Wednesday, 13 October 2021
Abstract: Clinical trials continue to determine whether therapeutic modulation of kinases can be used to suppress the immune system in autoimmune diseases or stimulate for antiviral and cancer immunotherapy. The discovery and characterization of small molecule modulators have aided these efforts. The Transcreener ADP² Kinase Assay meets this need as it relies on direct ADP
- Published in Emerging Targets, HTS Assays, Innate Immunity