Researchers Use Transcreener Assay to Identify New PDE5 Inhibitor
Monday, 24 July 2023
by Bellbrook Labs
Phosphodiesterase 5 (PDE5) is most familiar in connection to sildenafil (Viagra), a popular pharmaceutical PDE5 inhibitor. In response to nitric acid-activated soluble guanylyl cyclase production of cGMP, dimeric PDE5 binds and hydrolyzes cGMP in vascular smooth muscles cells, lung, kidney, brain, cardiac myocytes, and platelets to create 5′-GMP. This is accomplished by cGMP binding to
- Published in Emerging Targets, HTS Assays, Products, Success Stories
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Transcreener ADP2 Assay Helps Uncover the Mechanics of Oncogenic PIK3CA Mutations
Monday, 26 June 2023
by Bellbrook Labs
The PIK3CA gene codes for the 110 kDa catalytic subunit (p110α) of phosphoinositide 3-kinase (PI3Ka). Together with its regulatory component, p85α (encoded by PIK3R), they comprise the powerful PI3Ka heterodimer. This heterodimer conducts growth impulses from multiple cell surface receptor tyrosine kinases (RTKs) to the interior of the cell. It is known that individual missense
- Published in Emerging Targets, HTS Assays, Success Stories
Transcreener® ADP2 Assay Delivers Precise Measurement of Functional MAPK14 Residence Time
Monday, 01 May 2023
by Bellbrook Labs
Researchers at the University of Tubingen use Transcreener to uncover how residence time works on a molecular level with p38α MAPK Inhibitors – Many factors affect the effectiveness of ligand-substrate or drug-target interactions. Conformational docking and stearic considerations help inform optimal “fits.” Ligand moiety solubility, both in free and bound forms, is also a consideration
- Published in Emerging Targets, HTS Assays, Innate Immunity, Products, Success Stories
Uncovering IRAK4 Inhibitors with a Transcreener Kinase Assay Kit
Friday, 23 October 2020
by Bellbrook Labs
Genentech discovers IRAK4 inhibitors using the Transcreener Kinase Assay Kit – IRAK is also known as Interluekin-1 Receptor Associated Kinase. There are four different proteins within this group, including IRAK1, IRAK2, IRAK3, and IRAK4. Each of these mediates interaction with MyD88 and its associated adaptor proteins. IRAK1 and IRAK4 are active enzymes that exhibit kinase
- Published in Success Stories
Researchers Validate Hsp70 Activity with the Transcreener ATPase Assay
Wednesday, 24 June 2020
by Bellbrook Labs
Newly expressed human Hsp70 activity now being studied – Heat shock protein 70 (Hsp70) is known for its upregulation during times of stress in order to maintain homeostasis. Like other heat shock proteins, Hsp70 is a chaperone protein that assists other proteins in correctly folding to become fully active molecules. It binds to damaged, misfolded,
- Published in HTS Assays, Success Stories
Transcreener Assay Used to Help Discover CPS1 Inhibitor
Tuesday, 12 May 2020
by Bellbrook Labs
Researchers look to discover a CPS1 inhibitor with an eye on cancer – Without properly functioning carbamoyl-phosphate synthetase I (CPS1), large amounts of highly toxic ammonia is able to accumulate in the body contributing to neurological conditions.2 Increased levels of the enzyme have been found in some cancers including lung adenocarcinoma (LADC).1 When overexpressed, CPS1
- Published in Success Stories
Transcreener Assay Used to Evaluate PI3K Inhibitors
Tuesday, 17 March 2020
by Bellbrook Labs
PI3K Inhibitors and Cancer – Although cancer is widespread, determining the culprit, as well as individual treatment, is challenging. A variety of cancer types exist with a multitude of contributors. Being the most common human genetic disease, a whole host of genes are involved. Some contribute to curbing cancer (tumor suppressor genes), while oncogenes promote
- Published in Success Stories
Transcreener ADP Assay Used to Discover RIPK1 Inhibitors
Tuesday, 18 February 2020
by Bellbrook Labs
Genentech discovers novel RIPK1 inhibitors – Like other serine/threonine protein kinases, receptor-interacting serine/threonine-protein kinase 1 (RIPK1) plays a role in several signaling pathways that keep us living healthy, functioning lives. RIPK1 is one of those enzymes’ that wears a variety of hats depending on what function needs attention. It contributes to cell death as well
- Published in Success Stories
Researchers Employ GTPase Assay to Investigate Rab Proteins
Thursday, 26 September 2019
by Bellbrook Labs
Measuring Rab interaction with a GTPase Assay – GTPases are in the limelight in biology and drug discovery because of their role in a wide range of cellular processes and functions. They are highly conserved regulatory proteins involved in such diverse roles as signal transduction, protein biosynthesis, cell adhesion, membrane remodeling and trafficking, cytoskeletal reorganization,
- Published in HTS Assays, Success Stories
ABC Transporter ATPase Assay Helps Discover MsbA Inhibitors
Tuesday, 16 July 2019
by Bellbrook Labs
MsbA Inhibitors Help Fight Against Gram-Negative Bacteria As pathogens continue to wreak havoc on human health, new therapeutics are needed. HTS compatible assays such as the Transcreener ABC Transporter ATPase Assay help pave the way to establish new treatments. Gram-negative bacterial pathogens pose grave dangers in clinical and environmental settings alike. They include foodborne disease
- Published in Emerging Targets, HTS Assays, Success Stories