ABC Transporter ATPase Assay Helps Discover MsbA Inhibitors

ABC Transporter ATPase Assay Inhibitor
MsbA Inhibitors Help Fight Against Gram-Negative Bacteria As pathogens continue to wreak havoc on human health, new therapeutics are needed. HTS compatible assays such as the Transcreener ABC Transporter ATPase Assay help pave the way to establish new treatments. Gram-negative bacterial pathogens pose grave dangers in clinical and environmental settings alike. They include foodborne disease
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Novartis Uses Lipid Kinase Assay in a Quest for a PI4K Inhibitor

Lipid Kinase Assay PI4K Inhibitor
Did you know that diarrhea is the second leading cause of death globally among children under the age of 5?1 Perhaps you have heard of one of the many diarrheal diseases such as cryptosporidiosis caused by a parasite called Cryptosporidium. Many people in the US might associate swimming pools with outbreaks of cryptosporidiosis due to
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Researchers Use ATPase Enzyme Assay to Study SMCHD1 Mutations

Performing an ATPase Enzyme Assay
An ATPase enzyme assay – for more than just HTS. Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) is an important protein that plays a role in gene regulation through altering DNA by addition of methyl groups.  It also contributes to the normal development of the nose, eyes, and other structures of the
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Transcreener ADP Assay Enables Evaluation of NIK Inhibitors in Lupus Drug Discovery

Lupus NIK Inhibitors
  Systemic lupus erythematosus (SLE), or lupus, takes a devastating toll. An autoimmune disease that triggers a dysfunctional immune system to attack its own tissues, SLE strikes young women most often — about 4 to 12 times more frequently than men — with women aged 15 to 45 being at highest risk. Both genetic and
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Acetyl-CoA Carboxylase Inhibitors: Transcreener ADP Assay Powers Drug Detection Strategy

Acetyl-CoA Carboxylase Inhibitors
The impact of Acetyl-CoA carboxylases (ACCs) on eukaryotic metabolism and metabolic-related disease states is profound. ACCs catalyze the formation of malonyl-CoA by ATP-dependent carboxylation of acetyl-CoA. In humans, the two isoforms of ACC exhibit highly regulated, tissue-specific patterns of expression, with ACC1 being present in lipogenic tissues such as liver and adipose, and ACC2 being
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Enhancing Checkpoint Blockade Immunotherapy: Sensitizing Tumors with HSP90 Inhibitors

HSP90 Inhibitor Immunotherapy
Checkpoint blockade is turning heads in the oncology community. This form of immunotherapy, which uses anti-PD-1 or anti-CTLA-4 antibodies to coax the immune system into recognizing and attacking cancer cells, has been a game-changer — but only for some patients. Types of cancer for which checkpoint blockade currently cannot presently be used include gastrointestinal tumors
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Minding the Membrane: Powerful Approach to Interrogate Molecular Events in Ras-PI3K Interactions

It has been 30 years since the lipid signaling molecule phosphatidylinositol 3,4,5-trisphosphate (PIP3) was first identified¹, and its role as a key regulator of cell growth and survival was recognized in the earliest investigations into this molecule.² Class IA phosphoinositide 3-kinases (PI3Ks) generate PIP3. The inappropriate activation of this pathway has grave consequences in many
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Transcreener Assay Helps Find New Approach to C. difficile

Clostridium difficile – One of the Deadliest Enemies in Hospitals There are half a million reasons to consider Clostridium difficile as “Enemy Number 1” for public health. That’s because the annual cases of C. difficile, or C diff, infection now top 500,000 per year in the U.S.—resulting in more than 29,000 deaths per year. Worse
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Transcreener ADP Assay Aids in Discovery of RIPK2 Inhibitors for Autoinflammation

When the body responds to a bacterial pathogen, fragments of bacterial peptidoglycan alert a human host to the presence of a microbial invader. A receptor named NOD2 recognizes the peptidoglycans and sounds the alarm, initiating immune responses by causing the production of pro-inflammatory cytokines via NF-κB and MAP kinase pathways 1. In this manner, NOD2
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Newly Patented Aurora A Kinase Inhibitor a Promising Contender for Cancer Treatment

Aurora A Kinase in Cancer Drug Development Aurora A Kinase, along with -B and -C, are members of a family of serine/threonine protein kinases that play critical roles in coordinating mitosis, including establishment of the mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. The enzyme’s role
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