The Transcreener GDP Assay determines GAP activity by measuring increases in GDP formation as GAP proteins stimulate the GTPase. By detecting GDP output, the assay provides a universal method to assess the activity of any GDP-producing enzyme in real-time.
Transcreener GAP Assay technology uses as simple but highly effective method that consists of an antibody selective to GDP and a far-red fluorescent tracer. GDP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The GAP assay is available with FP, FI, and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also make it extremely amiable to HTS.