|Next up in our BellBrook Labs Webinar Series:||Register Now!|
Transcreener® HTS Assays for UDP, GDP, and CMP as a Universal Glycosyltransferase Assay Platform
BellBrook’s Transcreener® HTS assays enable robust screening and profiling of glycosyltransferases using mix-and-read assays for immunodetection of UDP, GDP, and CMP nucleotide products using FP, FI, or TR-FRET readouts. On February 25 at 11 AM CST, our senior application scientist, Meera Kumar, will describe how the Transcreener® HTS assays can be used for direct detection of diverse human and microbial glycosyltransferases by measuring inhibitor dose responses.
See details online about our NEW! Transcreener® UDP2 TR-FRET Assay, or any of our Transcreener® HTS assays, the only HTS-compatible method for glycosyltransferases that allows direct detection of reaction products without the use of coupling enzymes.
Glycosyltransferase (GT) enzymes participate in extremely diverse metabolic and regulatory roles by catalyzing the transfer of sugar molecules to protein, lipid and carbohydrate acceptors as well as endogenous and xenobiotic small molecules. GTs are of increasing of interest as therapeutic targets in inherited metabolic disorders, cancer, and for antimicrobials.
From an HTS assay perspective, glycosyltransferases are a challenging target class because of the diversity of both donor and acceptor substrates. Most GT screening efforts have relied on either enzyme-coupled assay methods to detect nucleotide products, which are prone to interference, or mass-spectrometry, which has limited throughput.
Most of the 200 glycosyltransferases in humans use one of nine sugar-nucleotides as donors, with GDP, UDP, or CMP as the nucleotide moiety. Transcreener® HTS assays enable direct, highly selective immunodetection of all three of these nucleotide products in a homogenous (mix-and-read) format with FP, FI, and TR-FRET readouts, thus they provide a universal platform for GT screening and profiling.
In this webinar you can learn:
- How to use Transcreener® HTS assays for direct detection of glycosyltransferases that use different sugar-nucleotide donors.
- How to measure enzyme activity and inhibitor dose responses for several GTs, including human fucosyltransferase, glucosylceramide synthase, galactose N-acetyltransferases, β1-4 galactosyltransferase, sialyltransferase and C. dificile Toxin B.
Don’t miss out! Register now and join us on Wednesday, February 25 at 11 AM CST. After you register online, you’ll receive a confirmation email containing all the information you need to attend. We look forward to seeing you then!