Join us Wednesday, May 14th 2014 at 11:00 AM CDT:
– Registration closed! –
Biochemical HTS assays are a critical component of drug discovery programs focused on kinases and other types of ATP-dependent enzymes, such as chaperonins and helicases. Assembling, evaluating and optimizing high quality enzymes and assay reagents can be a costly and time consuming undertaking. The shift toward screening focused libraries against multiple targets in parallel can add to the assay development burden substantially.
In this webinar you will learn:
– How Transcreener assays work: homogenous immunodetection of nucleotides with far red fluorescent readouts.
– Why highly selective immunodetection of ADP is advantageous over more complex methods that rely on coupling enzymes to convert ADP to a detectable product.
– How monitoring ADP production in real time allows optimization of multiple assay parameters using a single reaction.
– How to obtain accurate IC50 values directly from raw Transcreener data, and eliminate the need for standard curves.
Participants should come away confident that they can apply a rapid, user friendly solution to screen or profile kinases and other types of ATP dependent enzymes with minimal pilot experimentation and reagent usage.
After registering, you will receive a confirmation email containing information about joining the webinar.