• Assay Selection Tool

BellBrook Labs

  • Products
    • Transcreener® HTS Assay Kits
      • Transcreener® ADP² Kinase Assay Kits
        • Transcreener® ADP2 Assay Kit – FP Readout
        • Transcreener® ADP2 Assay Kit – FI Readout
        • Transcreener® ADP2 Assay Kit – TR-FRET Readout
      • Transcreener® ADO CD73 Assay Kit
      • Transcreener® AMP²/GMP² Phosphodiesterase Assay Kits
        • Transcreener® AMP2/GMP2 FP Assay
        • Transcreener® AMP2/GMP2 Assay Kit – TR-FRET Readout
      • Transcreener® cGAMP cGAS Assay Kits
        • Transcreener® cGAMP Assay Kit – FP Readout
        • Transcreener® cGAMP Assay Kit – TR-FRET Readout
      • Transcreener dAMP Exonuclease Assay Kit
      • Transcreener® EPIGEN SAH Methyltransferase Assay Kit
      • Transcreener® GDP GTPase Assay Kits
        • Transcreener® GDP Assay Kit – FP Readout
        • Transcreener® GDP Assay Kit – FI Readout
        • Transcreener® GDP Assay Kit – TR-FRET Readout
      • Transcreener® UDP² Glycosyltransferase Assay Kits
        • Transcreener® UDP2 Assay Kit – FP Readout
        • Transcreener® UDP2 Assay Kit – FI Readout
        • Transcreener® UDP2 Assay Kit – TR-FRET Readout
      • Transcreener® 2-5A OAS Assay Kit
    • AptaFluor® HTS Assay Kits
      • AptaFluor® SAH Methyltransferase Assay Kit
    • Enzyme Assay Systems
      • TREX1 Assay System
    • Recombinant Enzymes
      • Human cGAS Enzyme
      • Mouse cGAS Enzyme
      • Human DDX3 Enzyme
      • Human OAS1 Enzyme
      • Human TREX1 Enzyme
    • Assay Plates
    • Ordering Information
  • Services
    • Assay Development Services
    • Lead Discovery Services
    • CD38 Assay Services
    • GTPase Profiling Services
  • Assays by Target
    • Kinase Assays
      • ADK Assays – Application
      • AMPK Assays – Application
      • IKK-beta Assays – Application
      • IRAK4 Assays – Application
      • JAK1 Assays – Application
      • JAK3 Assays – Application
      • MAPK8 Assays – Application
      • PKR Assays – Application
      • RIPK1 Assays – Application
      • TBK1 Assays – Application
    • GTPase Assays
      • GAP Assays – Application
      • GEF Assays – Application
      • KRAS Assays – Application
      • HRAS Assays – Application
      • NRAS Assays – Application
      • RRAS Assays – Application
      • Rac1 Assays – Application
      • RhoA Assays – Application
      • RhoC Assays – Application
      • Cdc42 Assays – Application
      • Ran Assays – Application
    • Methyltransferase Assays
      • EZH2 Assays – Application
      • G9a Assays – Application
      • SET7/9 Assays – Application
      • SET8 Assays – Application
      • PRMT1 Assays – Application
      • PRMT3 Assays – Application
      • PRMT4 Assays – Application
    • STING Pathway Assays
      • cGAS Assay Kits
      • ENPP1 Assays – Application
      • TREX1 Assay System
      • IKK-beta Assays – Application
      • TBK1 Assays – Application
    • Nucleotidase Assays
      • CD38 Assay Services
      • CD39 Assays – Application
      • CD73 Activity Assay Kits
    • Helicase / ATPase Assays
      • DDX3 Assays – Application
      • NSP13 Assays – Application
      • P97 Assays – Application
    • Glycosyltransferase Assays
      • Toxin B Assays – Application
      • GALNT2 Assays – Application
      • GALNT3 Assays – Application
      • BGalT1 Assays – Application
    • Phosphodiesterase Assays
      • PDE3 Assays – Application
      • PDE4 Assays – Application
      • PDE5 Assays – Application
      • PDE7 Assays – Application
    • Ligase and Synthetase Assays
      • SUMO E1 Assays – Application
      • Acyl CoA Synthetase Assays – Application
      • S-Acetyl CoA Synthetase Assays – Application
    • Exonuclease Assays
      • TREX1 Assay System
    • OAS Assays
      • OAS1 Assay Kits
    • Other Enzyme Assays
      • NUDT5 Assays – Application
  • Resources
    • Technical Manuals
    • Transcreener® Assays – Instrument Compatibility
    • Application Notes
    • Posters and Presentations
    • Publications
    • Transcreener® FAQ’s
    • Guides
      • Residence Time Guide
      • Hit Prioritization Guide
      • Kinases in Innate Immunity
  • Company
    • President’s Message
    • International Distributors
    • Careers
    • Downloads
    • Contact Us
  • Blog
  • MY CART
    No products in cart.

A Kinase Assay Kit to Interrogate Innate Immunity Targets

by Bellbrook Labs / Thursday, 15 April 2021 / Published in HTS Assays, Products
Performing a Kinase Assay Kit in a Lab

Kinases are essential players in cellular functions such as cell signaling, cellular transport, and protein regulation. For these reasons, many kinases are innate immunity targets. A kinase assay kit is ideal whether used for drug screening and dose-response measurements or detection when studying a particular cellular pathway. A universal ADP assay allows researchers to screen virtually any kinase target with one assay technology.

The human immune response is incredible and incredibly complex. It acts quickly and is considered the first line of defense against pathogens and foreign DNA from sources such as tumor cells. The innate immune response relies on several cell types and a multitude of proteins such as interferons (INFs) to be effective. This group of cytokines consists of three types and facilitates the removal of viral pathogens. Other types of cytokines include chemokines, interleukins, lymphokines, and tumor necrosis factors (TNF).  Since cytokines are involved in multiple cell signaling pathways, an imbalance can contribute to various illnesses.

As a first-line defense against illnesses, the body’s inflammatory response is activated. Inflammation is a necessary step in the immune response, allowing our bodies to fight and eliminate disease, allergies, and injuries. The inflammatory response recruits several mediators to aid in the destruction of whatever pathogen caused the reaction in the first place. However, even this natural response can lead to disease if not regulated.

Cytokines are responsible for driving and regulating the immune response. An imbalance can contribute to excessive inflammation. Janus kinases (JAK) are tyrosine kinases that activate cytokines by offering a phosphate group, taking from ATP leaving ADP.2 JAKs are just one group of kinases of many involved in the innate immune response that can be exploited for therapeutic purposes. Others that have been examined are AMP-activated kinase (AMPK), TANK Binding Kinase 1 (TBK1), and IκB Kinase (IKK), to name a few.1

A Kinase Assay Kit to Interrogate Innate Immunity Targets

Researchers have already started discovering hundreds of kinase inhibitors to combat a plethora of illnesses, including cancer and inflammatory-related disease; however, there is much to learn and even more potential for drug discovery. The Transcreener® ADP² Kinase Assay can detect the enzymatic activity of Kinases, ATPases, and virtually any enzyme that produces ADP.

Making the assay unique, it detects kinase activity, including serine/threonine protein kinases, making it ideal for inhibitor selectivity profiling.  The easy-to-use universal ADP kinase assay kit limits the need for specific substrate selective reagents.

As complex as the human immune response is and we continue to discover pathways and proteins involved that add to the complexity, researchers likewise persist in finding treatments for the many diseases caused by a compromised immune system.

Check Out BellBrook’s Application Note: Targeting Kinases Involved in the Innate Immune Response

Get the App Note

References

  1. Targeting Kinases Involved in the Innate Immune Response Using the Transcreener M Meera Kumar, Justin Brink, and Robert G . Lowery ADP. (2007), 8.
  2. Gaestel, M., Kotlyarov, A., & Kracht, M. (2009). Targeting innate immunity protein kinase signaling in inflammation. Nature Reviews Drug Discovery, 8(6), 480–499. https://doi.org/10.1038/nrd2829
Tagged under: Kinase Assay Kit, Transcreener ADP Kinase Assay

What you can read next

Webinar: Streamline HTS and Lead Discovery with the Transcreener ADP Kinase Assay
PARG in Human Immunity
Discovering the Emerging Importance of PARG in Immunity
Could an Inhibitor of Phosphodiesterase Activity be a Treatment for Alzheimer’s?

Categories

  • Company
  • Emerging Targets
  • Epigenetics
  • HTS Assays
  • Innate Immunity
  • Neurodegenerative Diseases
  • News
  • Products
  • Resources
  • Success Stories
  • Uncategorized

Recent Posts

  • SLAS 2023 Conference Exhibitor Announcement

    SLAS 2023 – HTS Assays and Discovery Services

    BellBrook Labs will exhibit and present posters...
  • BTK's Involved in Systemic lupus erythematosus

    The Challenging Search for BTK Inhibitors

    Bruton’s Tyrosine Kinase (BTK) is a 76kDa...
  • SARM1 Causes Axonal Death

    SARM1 Forefronts Research into Major Neurological Diseases

    SARM1 [Sterile alpha & toll/interleukin rec...
  • SIRT2 Affects Neurodegenerative Diseases

    SIRT2 Isoforms in Neurodegenerative Diseases & Cancer

    Silent information regulator type 2 (Sirtuin 2 ...
  • Patient With WRN Helicase Syndrome

    Can WRN Helicase Inhibitors Treat MSI-H Cancers?

    Over 100 years ago, Otto Werner first character...

Archives

BellBrook Labs
5500 Nobel Drive, Suite 230
Madison, Wisconsin 53711 USA
(608) 443-2400

info@bellbrooklabs.com

 Copyright © 2022 BellBrook Labs | All Rights Reserved | Privacy Policy | Terms of Use | FCOI | Sitemap

TOP