The ABL1 gene is a special type of gene that encodes a protein tyrosine kinase. It is involved in various cellular processes, including but not limited to cell adhesion, cell division, and cell differentiation. This protein’s activity is adversely regulated by its SH3 domain, which involves the deletion of the region that encodes the domain results of the oncogene. It has DNA-binding activity regulated by CDC2-mediated phosphorylation. This suggests the function of a cell cycle. The gene is commonly found in partner genes in many types of leukemia.
How a Kinase Assay Kit Assists in Undercovering Novel Inhibitors
Kinase assay kits are used to analyze enzymes, determine their activity, and find inhibitors, which is crucial when it comes to drug development related to leukemia and other cancers. Kinase activity assays offered by Bellbrook Labs require up to 10 times less enzyme than traditional methods. This means that you can save significant amounts of money on large screens.
In addition to saving money by using a smaller concentration of enzymes, the process can also save the researcher significant amounts of time. Instead of developing an assay for every single member of an enzyme family, the kinase assay uses one set of reagents to detect the ADP product common in kinase reactions.
Comparison Between Coupled Assay Methods and Transcreener Method
When you use acoupled assay method, you start with one coupling enzyme, then you add a second coupling enzyme, and sometimes a third. At that point the reporter enzyme leads to the signal. However, when using the Transcreener method, the detection is direct with only one step required.
Other methods need complex assay schemes that might include three or more steps to order to turn ADP into a signal that is detectable. Each enzyme in a cascade of detection is a potential target for the screened compounds. This can lead to far more false positives or even lead to missing a hit. This adds to the amount of compounds required in a counter-screen.