Our recorded webinar for Screening GAPs and GEFs with the Transcreener® GDP Assay is now available for viewing anytime
In Interrogating GTPase Effector Proteins: Screening GAPs and GEFs with the Transcreener® GDP Assay, a recorded webinar presented by BellBrook Labs scientist Meera Kumar, you can learn about our HTS-compatible Transcreener® GDP Assay methods for detection of GTPase-accelerating proteins (GAPs) and guanine nucleotide exchange factors (GEFs) based on their ability to stimulate steady state GTPase activity under appropriate conditions.
The one-hour webinar recording covers how Transcreener® GDP Assays:
- Enable robust screening and profiling of GTPases using direct detection of GDP with FP, FI or TR-FRET readouts.
- Measure GTPase-GEF catalytic activity based on stimulation of steady state GDP formation, using two DOCK family Rho GEFs with their respective Rho GTPase substrates as case studies.
- Offer a robust HTS method for identifying inhibitors of members of the “regulator of G-protein signaling” (RGS) superfamily that act as GAPs for heterotrimeric G-protein alpha subunits.
The Transcreener® GDP assays are the only method that allows direct detection of GDP without the use of coupling enzymes.