• Assay Selection Tool

BellBrook Labs

  • Products
    • Transcreener® HTS Assays
      • Transcreener® ADP² Kinase Assay
      • Transcreener® ADO CD73 Assay
      • Transcreener® AMP²/GMP² Phosphodiesterase Assay
      • Transcreener® cGAMP cGAS Assay
      • Transcreener® GDP GTPase Assay Kits
      • Transcreener® UDP² Glycosyltransferase Assay
      • Transcreener® EPIGEN SAH Methyltransferase Assay
    • AptaFluor® HTS Assays
      • AptaFluor® SAH Methyltransferase Assay
    • HTS Assays by Target Family
      • Kinase Assay Kits
      • ATPase Assay Kits
      • Glycosyltransferase Assay Kits
      • GTPase Assay Kits
        • GAP Assay Kits
        • GEF Assay Kits
      • Methyltransferase Assay Kits
      • Ligase and Synthetase Assay Kits
      • Phosphodiesterase Assay Kits
    • HTS Assays by Target
      • AMPK Assay Kits
      • cGAS Assay Kits
      • CD39 Assay Kits
      • CD73 Assay Kits
      • ENPP1 Assay Kits
      • EZH2 Assay Kits
      • IKK-beta Assay Kits
      • JAK1 Assay Kits
      • JAK3 Assay Kits
      • TBK1 Assay Kits
    • Recombinant cGAS Enzyme
    • Assay Plates
    • Ordering Information
  • Services
    • Assay Development Services
    • Lead Discovery Services
    • GTPase Profiling Services
    • OAS1 Assay Services
  • Innate Immunity
    • cGAS Activity Assays
    • CD73 Activity Assays
    • CD39 Activity Assays
    • ENPP1 Activity Assays
    • IKK-beta Activity Assays
    • JAK1 Activity Assays
    • JAK3 Activity Assays
    • OAS1 Activity Assays
    • TBK1 Activity Assays
  • Resources
    • Application Notes
    • Transcreener® Assays – Instrument Compatibility
    • Posters and Presentations
    • Publications
    • Technical Manuals
    • Transcreener® FAQ’s
    • Guides
      • Residence Time Guide
      • Hit Prioritization Guide
  • Company
    • President’s Message
    • International Distributors
    • Careers
    • Downloads
    • Contact Us
  • Blog
  • MY CART
    No products in cart.

New Transcreener UDP2 Assay with a Fluorescence Intensity Readout

by Bellbrook Labs / Friday, 29 May 2020 / Published in HTS Assays, News, Products
Transcreener Glycosyltransferase Assay Blog

BellBrook Labs scientists have developed a new universal UDP detection assay for the measurement of Glycosyltransferase activity.  The Transcreener UDP2 FI Assay extends the Transcreener platform for UDP detection by utilizing a simple fluorescence intensity (FI) output. It can be used on fluorescence readers typically found in academic and therapeutic research laboratories, as well as more complex multimode plate readers used in core facilities and HTS laboratories.

The assay employs a competitive FI method, which was designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. Enzyme activity is signaled by an increase in fluorescence intensity as the bound tracer is displaced from the Transcreener UDP2 Antibody-Quencher. The AlexaFluor® 594 tracer uses the same plate reader settings as commonly used Texas Red. FI can provide faster read times than FP or TR-FRET. The assay is a simple single-step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 100 µM.

UDP FI Glycosyltransferase Assay Schematic

The Transcreener UDP2 FI Assay is a universal biochemical HTS assay for enzymes that produce UDP, including:

  • Glycosyltransferase
  • Galactosyltransferase
  • Glucuronyltransferase
  • N-acetylglucosamyltransferase
  • N-acetlygalactosyltransferase
  • Xylosyltransferase
  • Glycogen, Cellulose, Lactose, and Hyaluronan Synthases

The Transcreener® UDP2 FI Assay provides the following benefits:

  • Accommodates UDP concentrations ranging from 1 µM to 100 µM.
  • Excellent data quality (Z’ ≥ 0.7) and signal at low substrate conversion using 1 µM UDP.
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Red tracer further minimizes interference from fluorescent compounds and light scattering.

Learn More About the Assay

Tagged under: Glycosyltransferase Activity Assay, Transcreener UDP Assay

What you can read next

Discovery on Target Conference 2016
A Specific Inhibitor for a Promiscuous Glycosyltransferase
BellBrook Labs Launches More Sensitive Transcreener® Assay for Glycosyltransferases

Categories

  • Company
  • Emerging Targets
  • Epigenetics
  • HTS Assays
  • Neurodegenerative Diseases
  • News
  • Products
  • Resources
  • Success Stories
  • Uncategorized

Recent Posts

  • SLAS 2021

    Join Us for SLAS 2021 Virtual Conference

    BellBrook Labs will be exhibiting at SLAS 2021,...
  • New cGAS TR-FRET Assay

    New TR-FRET Readout Now Available with the Transcreener cGAMP cGAS Assay

    Launched in November 2019, the Transcreener cGA...
  • Webinar Graphic

    Previously Recorded Webinar – Streamline cGAS Inhibitor Discovery with the Transcreener® cGAS Assay

    The immune system uses the recognition of forei...
  • Lab IRAK4 Inhibitors Kinase Assay Kit

    Uncovering IRAK4 Inhibitors with a Transcreener Kinase Assay Kit

    Genentech discovers IRAK4 inhibitors using the ...
  • Assay Development Services - Two Scientists Working In the Lab

    Five Considerations When Choosing a Biochemical Assay Development Services CRO

    There are many options out there for choosing a...

Archives

BellBrook Labs
5500 Nobel Drive, Suite 230
Madison, Wisconsin 53711 USA
(608) 443-2400

info@bellbrooklabs.com

 Copyright © 2019 BellBrook Labs | All Rights Reserved | Privacy Policy | Terms of Use | FCOI | Sitemap

TOP