Full-Length, Active, Human, Recombinant cGAS Enzyme

Active, full-length Human Cyclic GMP-AMP synthase enzyme, His-tagged protein expressed and purified from E.coli. The enzyme has been thoroughly validated with Transcreener cGAS Activity Assays. For specific activity, please refer to the Certificate of Analysis for each individual enzyme lot. Purity is >90% in a 20mM Tris 7.5, 200mM NaCl, 1mM DTT, 10% glycerol buffer composition. The enzyme is currently used in the Transcreener cGAS Activity Assay at 30 nM, which is 1.75 μg/mL (17.5 ng in 10 µL enzyme reaction volume in a 384-well format). Please adjust the enzyme concentration based on your requirements.

cGAS Enzyme Ordering Information

* For custom or bulk orders (over 100 µg) please contact us (info@bellbrooklabs.com) for a quote.

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Cat. No.	Product Description	Price	Add to Cart
2227	Recombinant cGAS Enzyme, Active, Human, Full-Length, 10 µg	$439.00	Add to Cart
2228	Recombinant cGAS Enzyme, Active, Human, Full-Length, 100 µg	$1,498.00	Add to Cart

Associated Transcreener cGAMP cGAS Assay Kits

* For custom or bulk orders (over 100,000 wells) please contact us (info@bellbrooklabs.com) for a quote.

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Cat. No.	Product Description	Price	Add to Cart
3024-1K	Transcreener cGAMP cGAS FP Assay (1,000 Assay, 384 Well)	$839.00	Add to Cart
3024-10K	Transcreener cGAMP cGAS FP Assay (10,000 Assay, 384 Well)	$4,849.00	Add to Cart
3024-100K	Transcreener cGAMP cGAS FP Assay (10 x 10,000 Assay, 384 Well)	$24,245.00	Add to Cart
3025-1K	Transcreener cGAMP cGAS TR-FRET Assay (1,000 Assay, 384 Well)	$919.00	Add to Cart
3025-10K	Transcreener cGAMP cGAS TR-FRET Assay (10,000 Assay, 384 Well)	$5,239.00	Add to Cart
3025-100K	Transcreener cGAMP cGAS TR-FRET Assay (10 x 10,000 Assay, 384 Well)	$26,195.00	Add to Cart

Learn More About Transcreener cGAS Activity Assays

Go to cGAS Assay Page

cGAS Screening Bundles

* Includes enough enzyme for respective wells when used at 1.75 μg/mL (30 nM)

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Cat. No.	Product Description	Price	Add to Cart
3024-1K-B	Includes Transcreener cGAS FP Assay 1K Kit and 20 µg Human cGAS Enzyme	$1,339.00	Add to Cart
3024-10K-B	Includes Transcreener cGAS FP Assay 10K Kit and 200 µg Human cGAS Enzyme	$6,999.00	Add to Cart
3025-1K-B	Includes Transcreener cGAS TR-FRET Assay 1K Kit and 20 µg Human cGAS Enzyme	$1,419.00	Add to Cart
3025-10K-B	Includes Transcreener cGAS TR-FRET Assay 10K Kit and 200 µg Human cGAS Enzyme	$7,349.00	Add to Cart

Certificates of Analysis

Cat. No. 2227 - 10 μg cGAS Enzyme

C of A for Lot# RD100B

C of A for Lot# TB3100C

Cat. No. 2228 - 100 μg cGAS Enzyme

C of A for Lot# RD100C

C of A for Lot# TB3100D

Alternate Names

Cyclic GMP-AMP synthase
cGAMP synthase
h-cGAS
2’3’-cGAMP synthase
Mab-21 domain-containing protein 1
C6orf150

Storage Information:

Store at -80ºC. Avoid freeze-thaws.

Learn More About Targeting the cGAS-STING Pathway Using the cGAS Enzyme in This Poster from BellBrook Labs.

Download the Poster

cGAS Enzyme Has an Important Role in the STING Pathway

When free DNA is in the cytoplasm of eukaryotic cells, it can be an indication of damage or infection to the cell. This DNA triggers an immune response led by type I interferon (IFN) production. The cGAS enzyme acts as a unique sensor for double-stranded DNA. When DNA binds to cGAS it activates the enzyme producing a dinucleotide cGAMP from ATP and GTP. cGAMP created by cGAS activates STING (stimulator of interferon genes), leading to IFN immune response in the cell.

Example cGAS Activity Under Initial Velocity Conditions

cGAS is activated by double-stranded DNA and requires ATP and GTP substrates for the production of cGAMP.

Full-Length Human cGAS Titration

Recombinant cGAS Enzyme