Transcreener ADP2 Adenosine Kinase Assay
The Transcreener ADP² Assay directly measures the ADP produced by Adenosine Kinase (also known as ADK or AK). These ADP measurements allow researchers to effectively determine the activity of the Adenosine Kinase enzyme. The assay provides a powerful tool to screen compound libraries for ADK modulators to help find new therapies for disease.
The kit comes complete with the detection reagents required to measure activity. Adenosine kinase enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of Adenosine Kinase As a Therapeutic Target
Adenosine Kinase is a cytosolic enzyme that acts to modulate levels of adenosine (ADO) in the cell. Extracellular ADO works as a messenger to localized tissue in response to damage and has a shielding effect on a wide range of conditions. The role of ADK in regulating ADO makes it a model candidate for potential therapeutics. Intracellular blocking of ADK has been shown to increase extracellular concentrations of ADO. ADK inhibitors have been tested for various therapies including inflammation, and neurological disorders. Other enzymes within the purinergic system that aid in the regulation of the ADO cascade include CD39 and CD73. (Jarvis, 2019)
Direct Detection of ADP to Measure ADK Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases