The Transcreener ADPR Assay is a biochemical assay that measures the production of ADPR (also known as ADP-ribose) in enzymatic reactions. It is designed specifically for high throughput screening (HTS), with a single-addition, mix-and-read format. As examples, this assay can be used to detect the activity of CD38 or PARG, both of which may have implications for several therapeutic indications.
The ADPR assay is easy to integrate into automated HTS workflows, with outstanding reagent stability, sensitive detection of ADPR down to 10 nM and as high as 50 μM, and compatibility with commonly used multimode plate readers.
The kit comes complete with detection reagents required to measure activity. Enzyme targets are not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme. CD38 enzyme and screening kits are available for separate purchase through BellBrook Labs.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.