Transcreener AMP2/GMP2 Phosphodiesterase Assay directly measures AMP or GMP produced by phosphodiesterase enzymes. These AMP/GMP measurements allow researchers to effectively determine the activity of the phosphodiesterase enzyme. The assay provides a powerful tool to screen compound libraries for phosphodiesterase modulators to help find new therapies for disease.
The kit comes complete with the detection reagents required to measure activity. Phosphodiesterase enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
Transcreener AMP2/GMP2 Assay kits allow detection of any AMP or GMP producing enzyme (e.g. ligases, synthetases, phosphodiesterase) using any precursor substrate, including cAMP, cGMP, ATP, or NAD. It is the only activity assay method for direct detection of unlabeled AMP or GMP; i.e. without coupling enzymes.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.