The Transcreener® AMP2/GMP2 Assay with FP readout measures AMP/GMP via antibodies selective to AMP/GMP and a far-red fluorescent tracer. AMP/GMP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any AMP/GMP-producing enzyme in a high throughput format.
The assay produces an excellent signal at low substrate conversion for a broad range of initial ATP concentrations (1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.