The Transcreener® AMP²/GMP² Assay kit with TR-FRET readout uses displacement of a tracer by AMP/GMP to measure enzymatic activity. This disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. 

The assay produces an excellent signal at low substrate conversions for a broad range of initial substrate concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. The universal detection method allows screening of virtually any AMP/GMP-producing enzyme in a high throughput format.