Case Study: Probing P97 with the Transcreener ADP² ATPase Assay
Discover ATPase Inhibitors with the Transcreener ADP2 Assay
Transcreener ATPase Assay Kits provide a sensitive, HTS capable platform as demonstrated in millions of wells and multiple publications. As a universal technology eliminating the need for laborious internal assay development, Transcreener directly measures ADP produced by enzymatic ATP hydrolysis. Direct detection provides a simple yet robust method, eliminating coupling enzymes while providing accurate results.
Ultra-Sensitive Assay Measures ATPase Activity
In this peer-reviewed article, learn how researchers at Cancer Research UK used the sensitivity of Transcreener as an HSP90 and HSP72 activity assay when the malachite green technique wasn't sensitive enough for their drug discovery.
ATPase Assay Principle - Direct ADP Detection with a Fluorescent Readout
ATPases are a group of enzymes often found in cell membranes that catalyzes the decomposition of ATP. As the enzyme hydrolyzes ATP, it yields a free phosphate ion, ADP, and energy. Sensitive detection of ADP by the Transcreener antibody displaces a tracer producing a fluorescent readout.
Three detection modes are available - you can choose between FP, FI or TR-FRET readouts. All three assays use far-red fluors, ensuring that interference from fluorescent library compounds and light scattering is reduced to insignificant levels.
There are a number of reasons for choosing one over the other: minimizing compound interference, maximizing instrument performance, comparison to previous data, or just familiarity and comfort level. Choose the one the best fits your research requirements.
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Applications
- Screen compound libraries for enzyme inhibitors
- Determine inhibitor selectivity between enzymes
- Profile hits for inhibitor potency
- Measure enzymatic activity
- Determine residence times for inhibitors
Fewer False Positives
Direct detection of ADP reduces the degree of interference seen with coupling enzyme-based assays, lowering the number of false positives. Far-red tracers eliminate fluorescent compound interference. Reduce your time triaging false hits and spend it finding druggable leads.
Non Radio-Active Assay Method
Transcreener provides an accurate, dependable, safe alternative to radio-labeled assays. No more disposal concerns.
Universal
The universal nature of Transcreener allows for the screening of virtually any ATPase enzyme. Universal assays also provide an effective technique for difficult targets. Get ahead of the curve with assays ready for emerging targets.
- Learn how Transcreener was used by Genentech as an ABC transporter activity assay.
- Researchers at UNC used the Transcreener platform as a RecA assay for HTS and follow up hit characterization.
Download Tutorial
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases
