The Transcreener CD39 Assay determines CD39 enzyme activity by directly measuring the AMP formed by the enzyme using the Transcreener AMP Assay. By detecting AMP output, the assay provides a universal method to assess the activity of any AMP-producing enzyme in real-time.
Transcreener CD39 Assay technology uses a simple but highly effective method that consists of an antibody selective to AMP and a far-red fluorescent tracer. AMP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The CD39 assay is available with FP and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS.