The Transcreener ADO CD73 Assay determines CD73 enzyme activity by using a coupling enzyme that converts ADO produced by CD73 to the products AMP and ADP. ADP can then be measured using the trusted Transcreener ADP2 Assay components. The assay provides a universal method to assess activity of any ADP-producing enzyme.
Transcreener CD73 Assay technology uses a simple but highly effective method that consists of an antibody selective to ADP over ATP and a far-red fluorescent tracer. ADP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The CD73 assay is available with FP readout. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS.