The Transcreener cGAMP cGAS Assay determines cGAS enzyme activity by directly measuring the cGAMP formed by the enzyme using the Transcreener cGAMP Assay. By detecting cGAMP output, the assay provides a universal method to assess the activity of any cGAMP-producing enzyme in real-time.
Transcreener cGAMP Assay technology uses a simple but highly effective method that consists of an antibody selective to cGAMP and a far-red fluorescent tracer. cGAMP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The cGAS assay is available with FP and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS.