The Transcreener® cGAMP Assay with FP readout measures cGAMP via antibodies selective to cGAMP and a far-red fluorescent tracer. cGAMP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any cGAMP-producing enzyme in a high throughput format.
The assay shows excellent data quality (Z' ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges from 0.5 μM to 100 μM. Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs.
Used in over millions of wells by pharma since 2006, Transcreener is an extensively validated enzyme detection method backed by the best technical support in the industry.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.