The Transcreener® cGAMP Assay with FP readout measures cGAMP via antibodies selective to cGAMP and a far-red fluorescent tracer. cGAMP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any cGAMP-producing enzyme in a high throughput format.
The assay shows excellent data quality (Z' ≥ 0.7) and signal (≥85 mP polarization shift) at cGAMP ranges from 0.5 μM to 100 μM. Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs.
Used in over millions of wells by pharma since 2006, Transcreener is an extensively validated enzyme detection method backed by the best technical support in the industry.