Direct Detection of cGAMP to Measure cGAS Enzymatic Activity

cGAS Assay Schematic FP

Screen for Novel cGAS Modulators

cGAMP Assay Pilot Screen

Achieve Robust Results

Results from cGAS Pilot Screen

Pilot screen from Life Chemicals Diversity Library. 1600 compounds screened with 10 nM cGAS enzyme and 100 µM ATP, 100 µM GTP, and 62.5 nm interferon stimulatory DNA. The enzyme reaction was 1 hour and plates were read on a BMG Pherastar® Plus plate reader.

Instrument Validation with Transcreener FP Assays

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier Instrument FP Assays
ADP2, AMP2/GMP2, GDP, UDP, EPIGEN SAH
bioteklogo TriStar²S LB 942 in review
Mithras² LB 943 in review
bioteklogo Cytation™ 5 validated
Cytation™ 3 validated
Cytation™ 1 validated
Synergy™ H1 validated
Synergy™ 2/H4/4 validated
Synergy™ HTX not capable
Synergy™ Neo 2 validated
BMGLABTECH Logo POLARstar® Omega validated
FLUOstar® Omega not capable
PHERAstar® FSX validated
PHERAstar® Plus/FS validated
CLARIOstar® validated
MDS AT logo Analyst® GT/HT validated
Gemini® XPS/EM not capable
SpectraMax® M2/M2e not capable
SpectraMax® M5/M5e/FlexStation® 3 not capable
SpectraMax® Paradigm validated
perkinElmerLogo EnVision®/EnVision® Xcite validated
tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated
Infinite® M200 not capable
Infinite® F500 validated
Infinite® F200/Ultraevolution contact us
Spark™ 10M validated

Validation Criteria

  • 384-Well Format
  • Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
  • Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
  • Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.

 

Transcreener cGAMP cGAS Assay Kits

* For custom or bulk orders (over 100,000 wells) please contact us (info@bellbrooklabs.com) for a quote.

In addition to our cGAMP cGAS Assay, Bellbrook Labs also offers the following products to aid in the drug discovery process:

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