Learn More About Transcreener Method Used for This DDX3 Assay
Discover DDX3 Modulators with the Transcreener ADP2 DDX3 Assay
The Transcreener ADP² Assay directly measures the ADP produced by DDX3 (also known as DEAD-box RNA Helicase 3 or DDX3 Helicase). These ADP measurements allow researchers to effectively determine the activity of the DDX3 enzyme. The assay provides a powerful tool to screen compound libraries for DDX3 modulators to help find new therapeutics for a variety of different conditions including antiviral therapies, autoimmune diseases, and cancer.
The kit comes complete with all the detection reagents required to measure activity. DDX3 enzyme is not included in the inhibitor screening assay kit. You can purchase the DDX3 enzyme from BellBrook Labs.
The Role of DDX3 As Therapeutic Target
DDX3 is part of the DEAD-box group of proteins which are a family of RNA helicases. These proteins have an active role in various cellular activities including innate immunity, viral reproduction, and tumorgenesis (Kukhanova, 2020). In some viruses such as HIV, DDX3 enables viral replication, while in others such as Dengue it has an anti-viral effect. The RNA helicase has similar effects on some cancer studies, wherein one type DDX3 is a tumor suppressor, while in another it acts as an enhancer. These qualities make DDX3 an interesting target for treating a variety of diseases.
DDX3 unwinds various forms of dsRNA, both 5' - 3' and 3' - 5'. It requires ATP and Mg²+ to unwind and releases RNA via ATP hydrolysis. The Transcreener ADP² Assay can then be used to measure the ADP produced in the reaction to measure enzymatic activity.
Direct Detection of ADP to Measure DDX3 Enzymatic Activity

The workhorse. Used in many large screens. Best deck and signal stability.

Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.

For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
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Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases