Transcreener® Inside…
The Transcreener® EPIGEN Assay provides universal methyltransferase detection in an HTS-proven format. It combines the extensively validated Transcreener® AMP²/GMP² Assay with coupling enzymes that convert the SAH produced in a Methyltransferase reaction to AMP.
- Universal: Detects any methyltransferase using any substrate
- Robust: Z’ values greater than 0.7 with low substrate consumption
- Homogenous Format: Simple mix-and-read format for HTS workflows
- Sensitive: Reduces enzyme usage and allows use of physiological SAM concentrations
- >24 hr reagent and signal stability: High quality data in automated workflows at any scale
- Validated: Optimized with a variety of enzymes including Reaction Biology’s purified MTases
Simple & HTS-Ready
The Transcreener® EPIGEN MT Assay is simple, HTS-ready and has the outstanding data quality and deck and signal stability that Transcreener® Assays are known for. Run your enzyme reaction, add the quench reagent, add detection reagents (coupling enzymes are pre-combined with Transcreener® reagents), and read your plates.
Detection of HMTs with the Transcreener® Epigen Methyltransferase Assay. Enzyme titrations of Set7/9, G9a, and Suv39H1 histone methyltransferases with SAM at the indicated concentrations and a Histone H3-derived peptide as acceptor substrate.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.
EPIGEN Methyltransferase Assays
| Cat. No. | Product Description | Price | Add to Cart |
|---|---|---|---|
| 3017-1K | Transcreener EPIGEN Methyltransferase Assay (1,000 Assay, 384 Well) | $1079.00 | Add to Cart |
| 3017-10K | Transcreener EPIGEN Methyltransferase Assay (10,000 Assay, 384 Well) | $6,289.00 | Add to Cart |
| 3017-100K | Transcreener EPIGEN Methyltransferase Assay (10 x 10,000 Assay, 384 Well) | $31,445.00 | Add to Cart |
