The Transcreener GALNT3 Assay determines GALNT3 enzyme activity by directly measuring the UDP formed by the enzyme using the Transcreener UDP Assay. By detecting UDP output, the assay provides a universal method to assess the activity of any UDP-producing enzyme in real-time.
Transcreener GALNT3 Assay technology uses a simple but highly effective method that consists of an antibody selective to UDP over UDP sugar donors and a far-red fluorescent tracer. UDP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout.
The GALNT3 assay is available with FP, FI, and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction add the detection reagent, and measure. The simplicity of the system yields robust results that also make it extremely amiable to HTS.