The Only Mix-and-Read Fluorescent GTPase Assay
The Transcreener GDP GTPase assay is a homogenous, fluorescent immunoassay that monitors the activity of GTPases along with GEF and GAP partners. The assay directly measures the amount of GDP produced in an in vitro GTPase reaction.
Learn More About Measuring GEF and GAP Activity:
Three Readouts Available
Three available fluorescent readouts provide a safe, HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than colorimetric phosphate detection methods such as malachite green. All readouts use far-red tracers to minimize compound interference and lower false-positive rates.
- Fluorescence polarization (FP)
- Fluorescence intensity (FI)
- Time-resolved Forster-resonance-energy-transfer (TR-FRET)
GTPase Assay Principle - Universal GDP Detection
GTP hydrolysis from GTPase activity creates the product GDP and an additional phosphate. GDP displaces tracer from a highly selective monoclonal antibody resulting in a decrease in fluorescence polarization and TR-FRET or an increase in fluorescence intensity - perfect for your high throughput screen!
What Can You Do With Transcreener
- Measure guanine nucleotide exchange factor (GEF) activity
- Measure GTPase-activating proteins (GAP) activity
- Detect any GDP producing enzyme: GTPases, Gα proteins, Ras-like G proteins (Ras, Roc, Rac, cdc42, etc.), fucosyltransferases, mannosyltransferases