The Transcreener GDP Assay Kit with FI readout measures GDP via antibodies selective to GDP and a fluorescent tracer. Enzymatically generated GDP displaces the tracer from an antibody-quencher conjugate, resulting in increased fluorescence. The universal detection method allows screening of virtually any GTPase in a high throughput format.
The Transcreener Assay offers reagents stability and compatibility with commonly used multimode plate readers. The FI GDP assay kit accommodates GTP concentrations ranging from 0.1 µM to 1,000 µM with excellent data quality (Z' ≥ 0.7) at low substrate conversion ( ≤10%).
Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated GDP detection method available backed by the best technical support in the industry.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.