The Only Mix-and-Read Fluorescent GTPase Assay …

The Transcreener® GDP FI Assay is a competitive immunoassay for GDP with a red fluorescent intensity (FI) readout. Designed specifically for HTS and featuring a single addition mix-and-read format and room temperature reagent stability, the Transcreener® GDP FI Assay is compatible with plate readers typically found in research labs as well as those used in HTS core facilities. The 3014-A kit is formatted for 96-well plates while the 3014-1K kit is formatted for 384-well and higher densities.

One Assay … Hundreds of Targets

  • GTPases
  • Fucosyltransferases
  • Mannosyltransferases

Universal HTS Assay

Homogenous, fluorescent assay for screening GTPases, fucosyltransferase or mannosyltransferase. Covers a broad range of GTP concentrations.

Sensitive

Highly selective, high affinity antibody enables nanomolar GDP detection in the presence of excess GTP.

Turnkey Protocol

Run your enzyme reaction, add Transcreener® detection reagents, read your plates.

Validated on Most Commercially Available Plate Readers

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier Instrument FI Assays
ADP2, GDP
bioteklogo TriStar²S LB 942 validated
Mithras² LB 943 validated
bioteklogo Cytation™ 5 validated
Cytation™ 3 validated
Cytation™ 1 validated
Synergy™ H1 validated
Synergy™ 2/H4/4 validated
Synergy™ HTX validated
Synergy™ Neo 2 validated
BMGLABTECH Logo POLARstar® Omega validated
FLUOstar® Omega validated
PHERAstar® FSX validated
PHERAstar® Plus/FS validated
CLARIOstar® validated
MDS AT logo Analyst® GT/HT validated
Gemini® XPS/EM validated
SpectraMax® M2/M2e validated
SpectraMax® M5/M5e/FlexStation® 3 validated
SpectraMax® Paradigm contact us
perkinElmerLogo EnVision®/EnVision® Xcite validated
tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated
Infinite® M200 validated
Infinite® F500 validated
Infinite® F200/Ultraevolution validated
Spark™ 10M validated

Validation Criteria

  • 384-Well Format
  • Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
  • Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
  • Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.

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