The Transcreener GDP Assay with FP readout measures GDP via antibodies selective to GDP and a far-red fluorescent tracer. GDP produced in the reaction competes with the tracer, decreasing fluorescent properties and providing a fluorescent readout. The universal detection method allows screening of virtually any kinase in a high throughput format.
The Transcreener Assay offers reagent stability and compatibility with commonly used multimode plate readers. The FP GDP Assay accommodates GTP concentrations ranging from 1 µM to 1,000 µM with excellent data quality (Z' ≥ 0.7) at low substrate conversion (typically 10-30%).
Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated GDP detection method available backed by the best technical support in the industry.