The Only Mix-and-Read Fluorescent GTPase Assay …

One Assay … Hundreds of Targets

  • GTPases
  • Fucosyltransferases
  • Mannosyltransferases

Universal HTS Assay

Homogenous, fluorescent assay for screening GTPases, fucosyltransferase or mannosyltransferase. Covers a broad range of GTP concentrations.

Sensitive

Highly selective, high-affinity antibody enables nanomolar GDP detection in the presence of excess GTP.

Turnkey Protocol

Run your enzyme reaction, add Transcreener® detection reagents, read your plates.

Note: Commercially available GTPase enzymes may contain GDP as a stabilizing agent. As the Transcreener® GDP FP Assay is based on the detection of GDP produced in an enzymatic reaction, these stabilizers will interfere with the assay

Validated on Most Commercially Available Plate Readers

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to ensure that Transcreener® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier Instrument FP Assays
ADP2, AMP2/GMP2, GDP, UDP, EPIGEN SAH
bioteklogo TriStar²S LB 942 in review
Mithras² LB 943 in review
bioteklogo Cytation™ 5 validated
Cytation™ 3 validated
Cytation™ 1 validated
Synergy™ H1 validated
Synergy™ 2/H4/4 validated
Synergy™ HTX not capable
Synergy™ Neo 2 validated
BMGLABTECH Logo POLARstar® Omega validated
FLUOstar® Omega not capable
PHERAstar® FSX validated
PHERAstar® Plus/FS validated
CLARIOstar® validated
MDS AT logo Analyst® GT/HT validated
Gemini® XPS/EM not capable
SpectraMax® M2/M2e not capable
SpectraMax® M5/M5e/FlexStation® 3 not capable
SpectraMax® Paradigm validated
perkinElmerLogo EnVision®/EnVision® Xcite validated
tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated
Infinite® M200 not capable
Infinite® F500 validated
Infinite® F200/Ultraevolution contact us
Spark™ 10M validated

Validation Criteria

  • 384-Well Format
  • Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
  • Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
  • Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.

 

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