The Transcreener® GDP Assay with TR-FRET readout uses displacement of a tracer by GDP to measure enzymatic activity. This disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
The assay produces an excellent signal at low substrate conversion for a broad range of initial GTP concentrations (0.1-1,000 µM). Both reagents & signal are stable at room temperature for over 24 hours, providing outstanding flexibility for automated high-volume screening programs. The universal detection method allows screening of virtually any GTPase in a high throughput format.
Used in over millions of well by pharma since 2006, Transcreener is the most extensively validated GDP detection method available backed by the best technical support in the industry.