Discover IRAK4 Modulators with the Transcreener ADP2 Assay
The Transcreener ADP² Kinase Assay directly measures the ADP produced by IRAK4 (also known as interleukin-1 receptor-associated kinase 4). These ADP measurements allow researchers to effectively determine the activity of the IRAK4 enzyme. The assay provides a powerful tool to screen compound libraries for IRAK4 modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases and cancer.
The kit comes complete with all the detection reagents required to measure activity. IRAK4 enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of IRAK4 As Therapeutic Target
As upstream toll-like receptors activate IRAK4, it phosphorylates IRAK1, stimulating downstream substrates by way of its kinase activity. This cascade leads to the activation of MAPKs and NF-κB and the production of pro-inflammatory cytokines. Further research supports IRAK4 as an innate immunity target. In these studies, IRAK knockout mice experienced little or no inflammation when stressed. It has also been found that patients with IRAK4 mutations have issues with pro-inflammatory cytokine and anti-inflammatory cytokine production.
Compounds that regulate IRAK4 provide a potential avenue for small molecule drugs to treat inflammatory and immune disorders. Some diseases associated with IRAK4 include cancer, lupus, inflammatory bowel disease, arthritis, and diabetes.
Learn how Genentech used the Transcreener ADP² Assay to discover IRAK4 inhibitors.
Direct Detection of ADP to Measure IRAK4 Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases