Discover JAK1 Modulators with the Transcreener ADP2 Assay
The Transcreener ADP² Kinase Assay directly measures the ADP produced by JAK1 (also known as Janus Kinase 1). By measuring the production of ADP researchers can effectively determine the activity of the JAK1 enzyme. The assay provides a powerful tool to screen compound libraries for JAK1 modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases and cancer.
The kit comes complete with all the detection reagents required to measure activity. JAK1 enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of JAK1 As Therapeutic Target
Signal transducer and activator of transcription (STAT) proteins are a family of transcription factors that through a chain of reactions ultimately lead to cellular immunity, proliferation, apoptosis, and differentiation. Once a cytokine binds to its receptor, JAK proteins are activated. JAKs in turn phosphorylate the cytokine receptor allowing for subsequent STAT binding which is then able to relocate to the nucleus where transcription occurs. In normal cells, this pathway enhances the immune response to fight unwanted diseases. Genome studies have found a multitude of polymorphisms in many of the proteins involved in this pathway which contribute to autoimmune and inflammatory disorders.
One current JAK1 inhibitor Upadacitinib (Rinvoq) is currently being marketed for Rheumatoid Arthritis treatment by AbbVie.
Direct Detection of ADP to Measure JAK1 Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases