Discover JAK3 Modulators with the Transcreener ADP2 Assay
The Transcreener ADP² Kinase Assay directly measures the ADP produced by JAK3 (also known as Janus Kinase 3). By measuring the production of ADP researchers can effectively determine the activity of the JAK3 enzyme. The assay provides a powerful tool to screen compound libraries for JAK3 modulators to help find new therapeutics for a variety of different conditions including autoimmune diseases and cancer.
The kit comes complete with all the detection reagents required to measure activity. JAK3 enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme.
The Role of JAK3 As Therapeutic Target
JAK3 is a tyrosine kinase that is activated by cytokine receptors. These cytokines include IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. JAK3 can also autophosphorylate in the presence of ATP; examples of that can be seen in the data below. JAK3 phosphorylates and activates STATs that induce the expression of genes in the cell nucleus.
Recent research has shown that JAK3 activation is common in many different cancers and that JAK3 autophosphorylation could be a rate-limiting step. JAK3 mutations have been identified in different forms of leukemia and lymphoma. Inhibiting JAK3 or JAK1 could provide a potential treatment for these cancers.
Because it is expressed only in hematopoietic cells, which should help to limit off-target effects, JAK3 is also being vetted for autoimmune diseases like rheumatoid arthritis. A JAK1 and JAK3 inhibitor, Tofacitinib is currently marketed by Pfizer. New specific JAK3 inhibitors could help limit the current off-target effects of the drug.
Direct Detection of ADP to Measure JAK3 Enzymatic Activity
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases