The Transcreener ADP2 Assay is a far-red, competitive assay that measures ketohexokinase (also known as KHK) activity via direct detection of ADP produced during an enzyme reaction. It is designed for high-throughput screening, with a single-addition, mix-and-read format. The assay is an excellent tool for researchers screening compound libraries to discover ketohexokinase modulators.
This ketohexokinase assay provides excellent data quality (Z' ≥ 0.7) and signal (≥ 85 mP polarization shift) at low substrate conversion (typically 10% or less). It accommodates ATP concentrations ranging from 0.1 µM to 1,000 µM.
The kit comes complete with the detection reagents required to measure activity. Ketohexokinase enzyme is not included in the inhibitor screening assay kit. Please contact us for questions related to acquiring the enzyme. This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.