- Transcreener® HTS Assays
- AptaFluor® HTS Assays
- HTS Assays by Target
- Ordering Information
Transcreener ADP2 Kinase Assay Kits rely on direct detection of ADP using a proprietary antibody that binds with exquisite specificity and affinity to ADP, with negligible cross-reactivity to ATP. This exquisite selectivity allows direct detection of ADP in the presence of an excess of ATP, a requirement for kinase activity assays run under initial velocity conditions (e.g., ≤10% ATP consumption). The high affinity of the ADP antibody means that the assay has the sensitivity for robust detection of ADP down to 10 nM with Z’ values of 0.7 or greater, so you can use less of your precious enzyme.
Other ADP detection methods rely on complex assay schemes that use three or more enzymatic steps to convert ADP into a detectable signal. Each of the enzymes in the detection cascade is a potential target for the compounds being screened, which increases the risk of false positives or of missing a hit, and requires additional wells for counter-screening.
Transcreener ADP2 Assays all use a homogeneous, mix and read assay format, which simplifies automation and provides maximal flexibility for assay protocols. Endpoint assays are typically used for HTS, and continuous assays are used for assay development or in determining residence times for kinase inhibitors.
During drug development initiatives, analysis of drug-target residence times can improve efficacy, increase therapeutic window, and reduce the risk of premature focus on candidate compounds that are likely to have undesirable side effects. This guide provides technical background on concepts and techniques for use of Transcreener biochemical assays to measure drug-target residence times, along with examples and case studies.
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Enter your contact info and learn how to determine drug-target residence times and improve your lead discovery with this 12-page guide!
The Transcreener ADP2 antibody and tracers are stable at room temperature for a minimum of 8 hours. This ensures that data quality does not suffer in situations where reagents are stored at room temperature prior to dispensing or if plates are not read immediately. The outstanding reagent and signal stability make Transcreener ADP2 Kinase Assays very reliable and easy to use in an automated HTS environment.
In addition to giving you flexibility of three detection modes, we have collaborated with the major suppliers of multimode readers, including Tecan, BMG, and Biotek to determine optimal instrument hardware and software settings for each of the Transcreener ADP2 Assays. Successful validation requires a Z’ of greater than 0.7 for ADP detection under initial velocity conditions (≤10% ATP conversion). Optimal filter sets and instrument settings are summarized in the technical manual for each Transcreener assay, and more detailed information is available in application notes. This ensures that whatever fluorescent detection mode or reader you prefer, you will get robust results with Transcreener reagents.
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