Transcreener Kinase Assay Principle
Transcreener assays are competitive immunoassays in a mix-and-read format. Displacement of tracer from the ADP antibody causes a change in its fluorescence properties. Transcreener is the simplest, most direct method for measuring ADP formed during a kinase reaction. Since the introduction of the first product in 2006, Transcreener has been used in millions of wells for HTS and lead discovery.
Biochemical kinase assay kits are available in far-red FP, FI, and TR-FRET detection modes to ensure optimal performance with your plate reader.
The workhorse. Used in many large screens. Best deck and signal stability.
Positive FI signal. Compatible with simple fluorescence plate readers. Faster read time than FP or TR-FRET.
For customers who prefer TR-FRET detection. Uses the same filter set as HTRF®.
Transcreener is Universal – Use with Virtually Any Kinase
Overcome the need for time-consuming, one-off development for individual members within an enzyme family by using a single set of reagents that detect the invariant product – ADP. The assay can be used with virtually any substrate including peptide substrates, protein substrates, and lipid substrates.
Example enzyme targets include:
- Protein kinases
- Lipid kinases
- Carbohydrate kinases
- Chaperonins (Hsp90, etc.)
Learn how Eli Lilly used Transcreener to discovery and patent Aurora A Kinase Inhibitors for cancer therapeutic development.
See how researchers at the University of Wisconsin - Milwaukee used the ADP assay to measure helicase activity in an effort to find small molecule inhibitors as a therapeutic for hepatitis C.
Discover how Pfizer used the assay to find Acetyl-CoA Carboxylase Inhibitors as potential treatments for a variety of indications including diabetes and metabolic syndrome.
- Screen for small molecule inhibitors
- Determine enzymatic activity
- Profile inhibitor potency/determine IC50 values
- Measure residence time/off-rate/dissociation of lead molecules with target
- Inhibitor selectivity profiling
- Mechanism of action studies
- Simple homogenous biochemical assay
- Direct detection of ADP eliminates laborious coupling steps
- Non-radioactive, safe assay method
- HTS Ready – Use in 96-well, 384-well, or 1536-well formats
- Choose from Fluorescent Polarization (FP), Fluorescent Intensity (FI), or time-resolved Förster-resonance-energy-transfer (TR-FRET) formats
- Sensitive, robust detection of ADP. This means up to 10X less enzyme, a significant cost reduction on large screens.
- Use with ATP concentrations from 0.1 to 1000 µM
- Far-red tracer further minimizes interference from fluorescent compounds and light scattering
Direct ADP Detection Minimizes Interference
Learn why direct ADP detection is the best HTS assay method for Kinases and ATPases