The Simplest, Most Direct ADP Detection Method Available … use for any kinase or ATPase.

Transcreener® Performance with a Positive Readout …

The Transcreener® ADP2 Fluorescent Intensity (FI) Kinase Assay produces a simple fluorescent intensity output which can be read on even the most basic fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers. It is a simple one-step homogenous detection assay, and is flexible with regard to ATP concentration (0.1 to 1,000 µM ATP). The assay provides excellent signal at low substrate conversion, with a Z’ > 0.7 at 2.5% ATP conversion using 1 µM ATP.

  • UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
  • SINGLE ADDITION: one-step, simple mix and read format.
  • SENSITIVE: Z’ > 0.7 at low ATP conversion.
  • POSITIVE FI READOUT: use with any fluorescence plate reader.

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance.  We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier Instrument FI Assays
ADP2, GDP
bioteklogo TriStar²S LB 942 validated
Mithras² LB 943 validated
bioteklogo Cytation™ 5 validated
Cytation™ 3 validated
Cytation™ 1 validated
Synergy™ H1 validated
Synergy™ 2/H4/4 validated
Synergy™ HTX validated
Synergy™ Neo 2 validated
BMGLABTECH Logo POLARstar® Omega validated
FLUOstar® Omega validated
PHERAstar® FSX validated
PHERAstar® Plus/FS validated
CLARIOstar® validated
MDS AT logo Analyst® GT/HT validated
Gemini® XPS/EM validated
SpectraMax® M2/M2e validated
SpectraMax® M5/M5e/FlexStation® 3 validated
SpectraMax® Paradigm contact us
perkinElmerLogo EnVision®/EnVision® Xcite validated
tecanLogo Infinite® M1000/M1000Pro/Safire2™ validated
Infinite® M200 validated
Infinite® F500 validated
Infinite® F200/Ultraevolution validated
Spark™ 10M validated

Validation Criteria

  • 384-Well Format
  • Z’-Factor ≥ 0.7 at 10% conversion of 10 μM ATP
  • Δ mP ≥ 95 mP at 10% conversion of 10 μM ATP
  • Z’ and Δ mP specifications to be met using Transcreener ADP2 Assay reagents
  • Read time to achieve Z’ and Δ mP specifications ≤ 5 minutes

Look for these stickers on instruments that have successfully met the validation criteria.

 

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