The Transcreener® ADP2 Assay Kit with FI readout measures ADP via antibodies selective to ADP and a fluorescent tracer. Enzymatically generated ADP displaces the tracer from an antibody-quencher conjugate, resulting in increased fluorescence. The universal detection method allows screening of virtually any kinase in a high throughput format.
The Transcreener FI ADP Assay Kit produces a simple fluorescent intensity output which can be read on even the most basic fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers. It is a simple one-step homogenous detection assay, and accommodates ATP concentrations from 0.1 to 100 µM ATP. The assay provides excellent signal at low substrate conversion, with a Z' ≥ 0.7 at 2.5% ATP conversion using 1 µM ATP.
Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry.
This assay is designed to be used with purified enzyme preparations. Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples.