The Transcreener® ADP2 FP Assay measures the progress of any enzyme that produces ADP. Displacement of the tracer by ADP causes a decrease in fluorescence polarization. The assay uses a red-shifted tracer, which reduces compound interference and provides a robust ratiometric readout.
- UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
- SINGLE ADDITION: one-step, simple mix and read format.
- HTS-READY: >24 hour deck and signal stability.
- SENSITIVE: Z’ > 0.7 at low ATP conversion.