Transcreener® ADP2 FP Assay

The Simplest, Most Direct ADP Detection Method Available. Use for any kinase or ATPase.

Transcreener^® ADP² FP Assay uses a highly sensitive antibody against ADP and a far-red tracer to produce an excellent signal at less than 10% ATP consumption for a broad range of initial ATP concentrations (0.1-1,000 µM). Both reagents and signal are stable at room temperature for over 24 hours providing outstanding flexibility for automated high-volume screening programs. Used in over millions of wells by pharma since 2006, Transcreener is the most extensively validated ADP detection method available backed by the best technical support in the industry.

The Transcreener^® ADP² FP Assay measures the progress of any enzyme that produces ADP. Displacement of the tracer by ADP causes a decrease in fluorescence polarization. The assay uses a red-shifted tracer, which reduces compound interference and provides a robust ratiometric readout.

UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
SINGLE ADDITION: one-step, simple mix and read format.
HTS-READY: >24 hour deck and signal stability.
SENSITIVE: Z’ > 0.7 at low ATP conversion.

Transcreener ADP² FP Assay Technical Manual (PDF)

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Cat. No.	Product Description	Price	Add to Cart
3010-1K	Transcreener ADP2 FP Assay (1,000 Assay, 384 Well)	$639.00	Add to Cart
3010-10K	Transcreener ADP2 FP Assay (10,000 Assay, 384 Well)	$3,689.00	Add to Cart
3010-100K	Transcreener ADP2 FP Assay (10 X 10,000 Assay, 384 Well)	$18,445.00	Add to Cart

The instrument validation program is a collaborative effort with the major suppliers of multimode readers to insure that Transcreener^® assays perform optimally on your specific instrument. Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. We provide you with application notes with all of the important details, so you can be confident of success using Transcreener assays on the instrument of your choice.

Supplier	Instrument	FP Assays ADP², AMP²/GMP², GDP, UDP, EPIGEN SAH
	TriStar²S LB 942	in review
	Mithras² LB 943	in review

	Cytation™ 5	validated
	Cytation™ 3	validated
	Cytation™ 1	validated
	Synergy™ H1	validated
	Synergy™ 2/H4/4	validated
	Synergy™ HTX	not capable
	Synergy™ Neo 2	validated

	POLARstar^® Omega	validated
	FLUOstar^® Omega	not capable
	PHERAstar^® FSX	validated
	PHERAstar^® Plus/FS	validated
	CLARIOstar^® /Plus	validated

	Analyst^® GT/HT	validated
	Gemini^® XPS/EM	not capable
	SpectraMax^® M2/M2e	not capable
	SpectraMax^® M5/M5e/FlexStation^® 3	not capable
	SpectraMax^® Paradigm	validated

	EnVision^®/EnVision^® Xcite	validated

	Infinite^® M1000/M1000Pro/Safire2™	validated
	Infinite^® M200	not capable
	Infinite^® F500	validated
	Infinite^® F200/Ultra^evolution	contact us
	Spark™ 10M	validated